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Journal of Nanomaterials
Volume 2012 (2012), Article ID 593471, 8 pages
Research Article

Quantitative Analysis and Efficient Surface Modification of Silica Nanoparticles

Department of Chemistry, Seoul National University, Seoul 151-747, Republic of Korea

Received 31 January 2012; Accepted 8 April 2012

Academic Editor: Hongchen Chen Gu

Copyright © 2012 Hak-Sung Jung et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aminofunctional trialkoxysilanes such as aminopropyltrimethoxysilane (APTMS) and (3-trimethoxysilylpropyl)diethylenetriamine (DETAS) were employed as a surface modification molecule for generating monolayer modification on the surface of silica (SiO2) nanoparticles. We were able to quantitatively analyze the number of amine functional groups on the modified SiO2 nanoparticles by acid-base back titration method and determine the effective number of amine functional groups for the successive chemical reaction by absorption measurements after treating with fluorescent rhodamine B isothiocyanate (RITC) molecules. The numbers of amine sites measured by back titration were 2.7 and 7.7 ea/nm2 for SiO2-APTMS and SiO2-DETAS, respectively, while the numbers of effective amine sites measured by absorption calibration were about one fifth of the total amine sites, namely, 0.44 and 1.3 ea/nm2 for SiO2-APTMS(RITC) and SiO2-DETAS(RITC), respectively. Furthermore, it was confirmed that the reactivity of amino groups on the surface-modified silica nanoparticles could be maintained in ethanol for more than 1.5 months without showing any significant differences in the reactivity.