Table of Contents Author Guidelines Submit a Manuscript
Journal of Nanomaterials
Volume 2012, Article ID 858073, 9 pages
http://dx.doi.org/10.1155/2012/858073
Research Article

In Vitro Biocompatibility of Endodontic Sealers Incorporating Antibacterial Nanoparticles

1Department of Endodontics, Hebrew University-Hadassah School of Dental Medicine, 91120 Jerusalem, Israel
2Department of Prosthodontics, Hebrew University-Hadassah School of Dental Medicine, 91120 Jerusalem, Israel
3Department of Periodontics, Hebrew University-Hadassah School of Dental Medicine, 91120 Jerusalem, Israel

Received 2 April 2012; Revised 12 June 2012; Accepted 14 June 2012

Academic Editor: Xiaoming Li

Copyright © 2012 Itzhak Abramovitz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. Kakehashi, H. R. Stanley, and R. J. Fitzgerald, “The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats,” Oral Surgery, Oral Medicine, Oral Pathology, vol. 20, no. 3, pp. 340–349, 1965. View at Google Scholar · View at Scopus
  2. D. J. Caplan, “Epidemiologic issues in the studies of association between apical periodontitis and systemic health,” Endodontic Topics, vol. 8, pp. 15–35, 2004. View at Publisher · View at Google Scholar
  3. L. B. Peters, P. R. Wesselink, and W. R. Moorer, “The fate and the role of bacteria left in root dentinal tubules,” International Endodontic Journal, vol. 28, no. 2, pp. 95–99, 1995. View at Google Scholar · View at Scopus
  4. J. L. Gutmann and D. E. Witherspoon, “Obturation of the cleaned and shaped root canal system,” in Pathways of the Pulp, S. Choen and R. C. Burns, Eds., Mosby, St. Louis, Mo, USA, 2002. View at Google Scholar
  5. U. Sjögren, D. Figdor, S. Persson, and G. Sundqvist, “Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis,” International Endodontic Journal, vol. 30, no. 5, pp. 297–306, 1997. View at Google Scholar · View at Scopus
  6. I. M. Saleh, I. E. Ruyter, M. Haapasalo, and D. Ørstavik, “Bacterial penetration along different root canal filling materials in the presence or absence of smear layer,” International Endodontic Journal, vol. 41, no. 1, pp. 32–40, 2008. View at Publisher · View at Google Scholar · View at Scopus
  7. I. Willershausen, A. Callaway, B. Briseño, and B. Willershausen, “In vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealers,” Head & Face Medicine, vol. 7, p. 15, 2011. View at Publisher · View at Google Scholar
  8. L. Peng, L. Ye, H. Tan, and X. Zhou, “Outcome of root canal obturation by warm gutta-percha versus cold lateral condensation: a meta-analysis,” Journal of Endodontics, vol. 33, no. 2, pp. 106–109, 2007. View at Publisher · View at Google Scholar · View at Scopus
  9. T. Schwarze, G. Leyhausen, and W. Geurtsen, “Long-term cytocompatibility of various endodontic sealers using a new root canal model,” Journal of Endodontics, vol. 28, no. 11, pp. 749–753, 2002. View at Google Scholar · View at Scopus
  10. L. G. Breault, G. S. Schuster, M. A. Billman et al., “The effects of intracanal medicaments, fillers, and sealers on the attachment of human gingival fibroblasts to an exposed dentin surface free of a smear layer,” Journal of Periodontology, vol. 66, no. 7, pp. 545–551, 1995. View at Google Scholar · View at Scopus
  11. A. Tamse, I. Kaffe, M. M. Littner, and A. Kozlovsky, “Paresthesia following overextension of AH-26: report of two cases and review of the literature,” Journal of Endodontics, vol. 8, no. 2, pp. 88–90, 1982. View at Publisher · View at Google Scholar · View at Scopus
  12. H. Lovschall, M. Eiskjaer, and D. Arenholt-Bindslev, “Formaldehyde cytotoxicity in three human cell types assessed in three different assays,” Toxicology in Vitro, vol. 16, no. 1, pp. 63–69, 2002. View at Publisher · View at Google Scholar · View at Scopus
  13. N. Beyth, Y. Houri-Haddad, L. Baraness-Hadar, I. Yudovin-Farber, A. J. Domb, and E. I. Weiss, “Surface antimicrobial activity and biocompatibility of incorporated polyethylenimine nanoparticles,” Biomaterials, vol. 29, no. 31, pp. 4157–4163, 2008. View at Publisher · View at Google Scholar · View at Scopus
  14. I. Yudovin-Farber, N. Beyth, A. Nyska, E. I. Weiss, J. Golenser, and A. J. Domb, “Surface characterization and biocompatibility of restorative resin containing nanoparticles,” Biomacromolecules, vol. 9, no. 11, pp. 3044–3050, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. J. M. Anderson and K. M. Miller, “Biomaterial biocompatibility and the macrophage,” Biomaterials, vol. 5, no. 1, pp. 5–10, 1984. View at Publisher · View at Google Scholar · View at Scopus
  16. K. M. Miller and J. M. Anderson, “In vitro stimulation of fibroblast activity by factors generated from human monocytes activated by biomedical polymers,” Journal of Biomedical Materials Research, vol. 23, no. 8, pp. 911–930, 1989. View at Google Scholar · View at Scopus
  17. I. Yudovin-Farber, N. Beyth, E. I. Weiss, and A. J. Domb, “Antibacterial effect of composite resins containing quaternary ammonium polyethyleneimine nanoparticles,” Journal of Nanoparticle Research, vol. 12, no. 2, pp. 591–603, 2010. View at Publisher · View at Google Scholar · View at Scopus
  18. D. A. Scudiero, R. H. Shoemaker, K. D. Paull et al., “Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines,” Cancer Research, vol. 48, no. 17, pp. 4827–4833, 1988. View at Google Scholar · View at Scopus
  19. A. S. Al-Hiyasat, M. Tayyar, and H. Darmani, “Cytotoxicity evaluation of various resin based root canal sealers,” International Endodontic Journal, vol. 43, no. 2, pp. 148–153, 2010. View at Publisher · View at Google Scholar · View at Scopus
  20. L. Kesavalu, J. L. Ebersole, R. L. Machen, and S. C. Holt, “Porphyromonas gingivalis-virulence in mice: induction of immunity to bacterial components,” Infection and Immunity, vol. 60, no. 4, pp. 1455–1464, 1992. View at Google Scholar · View at Scopus
  21. I. Frolov, Y. Houri-Hadad, A. Soskolne, and L. Shapira, “In vivo exposure to Porphyromonas gingivalis up-regulates nitric oxide but suppresses tumour necrosis factor-α production by cultured macrophages,” Immunology, vol. 93, no. 3, pp. 323–328, 1998. View at Publisher · View at Google Scholar · View at Scopus
  22. American Association of Endodontics—position paper, Concerning Paraformaldehyde-Containing Endodontic Filling Materials and Sealers, A.A.E. Endodontics, Chicago, Ill, USA, 1988.
  23. W. Geurtsen, “Biocompatibility of root canal filling materials,” Australian Endodontic Journal, vol. 27, no. 1, pp. 12–21, 2001. View at Google Scholar · View at Scopus
  24. A. D. Wilson, D. J. Clinton, and R. P. Miller, “Zinc oxide-eugenol cements. IV. Microstructure and hydrolysis,” Journal of Dental Research, vol. 52, no. 2, pp. 253–260, 1973. View at Google Scholar · View at Scopus
  25. P. Brodin, “Neurotoxic and analgesic effects of root canal cements and pulp-protecting dental materials,” Endodontics & dental traumatology, vol. 4, no. 1, pp. 1–11, 1988. View at Google Scholar · View at Scopus
  26. A. Samara, Y. Sarri, D. Stravopodis, G. N. Tzanetakis, E. G. Kontakiotis, and E. Anastasiadou, “A comparative study of the effects of three root-end filling materials on proliferation and adherence of human periodontal ligament fibroblasts,” Journal of Endodontics, vol. 37, no. 6, pp. 865–870, 2011. View at Publisher · View at Google Scholar · View at Scopus
  27. M. R. Leonardo, L. A. B. D. Silva, M. T. Filho, and R. S. D. Silva, “Release of formaldehyde by 4 endodontic sealers,” Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, vol. 88, no. 2, pp. 221–225, 1999. View at Google Scholar · View at Scopus
  28. H. Ersev, G. Schmalz, G. Bayirli, and H. Schweikl, “Cytotoxic and mutagenic potencies of various root canal filling materials in eukaryotic and prokaryotic cells in vitro,” Journal of Endodontics, vol. 25, no. 5, pp. 359–363, 1999. View at Google Scholar · View at Scopus
  29. J. D. Kettering and M. Torabinejad, “Cytotoxicity of root canal sealers: a study using HeLa cells and fibroblasts,” International Endodontic Journal, vol. 17, no. 2, pp. 60–66, 1984. View at Google Scholar · View at Scopus
  30. J. Heil, G. Reifferscheid, P. Waldmann, G. Leyhausen, and W. Geurtsen, “Genotoxicity of dental materials,” Mutation Research, vol. 368, no. 3-4, pp. 181–194, 1996. View at Publisher · View at Google Scholar · View at Scopus
  31. B. I. Cohen, M. K. Pagnillo, B. L. Musikant, and A. S. Deutsch, “Formaldehyde evaluation from endodontic materials,” Oral health, vol. 88, no. 12, pp. 37–39, 1998. View at Google Scholar · View at Scopus
  32. J. Volk, J. Engelmann, G. Leyhausen, and W. Geurtsen, “Effects of three resin monomers on the cellular glutathione concentration of cultured human gingival fibroblasts,” Dental Materials, vol. 22, no. 6, pp. 499–505, 2006. View at Publisher · View at Google Scholar · View at Scopus
  33. M. A. Versiani, J. R. Carvalho, M. I. A. F. Padilha, S. Lacey, E. A. Pascon, and M. D. Sousa-Neto, “A comparative study of physicochemical properties of AH Plus and Epiphany root canal sealants,” International Endodontic Journal, vol. 39, no. 6, pp. 464–471, 2006. View at Publisher · View at Google Scholar · View at Scopus
  34. M. Kaga, M. Noda, J. L. Ferracane, W. Nakamura, H. Oguchi, and H. Sano, “The in vitro cytotoxicity of eluates from dentin bonding resins and their effect on tyrosine phosphorylation of L929 cells,” Dental Materials, vol. 17, no. 4, pp. 333–339, 2001. View at Publisher · View at Google Scholar · View at Scopus
  35. H. H. Chang, M. K. Guo, F. H. Kasten et al., “Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA,” Biomaterials, vol. 26, no. 7, pp. 745–753, 2005. View at Publisher · View at Google Scholar · View at Scopus
  36. Y. C. Ho, F. M. Huang, and Y. C. Chang, “Mechanisms of cytotoxicity of eugenol in human osteoblastic cells in vitro,” International Endodontic Journal, vol. 39, no. 5, pp. 389–393, 2006. View at Publisher · View at Google Scholar · View at Scopus
  37. F. M. Huang, S. F. Yang, and Y. C. Chang, “Effects of root canal sealers on alkaline phosphatase in human osteoblastic cells,” Journal of Endodontics, vol. 36, no. 7, pp. 1230–1233, 2010. View at Publisher · View at Google Scholar · View at Scopus
  38. M. G. Brackett, A. Marshall, P. E. Lockwood et al., “Cytotoxicity of endodontic materials over 6-weeks ex vivo,” International Endodontic Journal, vol. 41, no. 12, pp. 1072–1078, 2008. View at Publisher · View at Google Scholar · View at Scopus
  39. B. Gao, X. Zhang, and Y. Zhu, “Studies on the preparation and antibacterial properties of quaternized polyethyleneimine,” Journal of Biomaterials Science, Polymer Edition, vol. 18, no. 5, pp. 531–544, 2007. View at Publisher · View at Google Scholar · View at Scopus
  40. N. Beyth, I. Yudovin-Farber, R. Bahir, A. J. Domb, and E. I. Weiss, “Antibacterial activity of dental composites containing quaternary ammonium polyethylenimine nanoparticles against Streptococcus mutans,” Biomaterials, vol. 27, no. 21, pp. 3995–4002, 2006. View at Publisher · View at Google Scholar · View at Scopus
  41. X. Li, C. A. van Blitterswijk, Q. Feng, F. Cui, and F. Watari, “The effect of calcium phosphate microstructure on bone-related cells in vitro,” Biomaterials, vol. 29, no. 23, pp. 3306–3316, 2008. View at Publisher · View at Google Scholar · View at Scopus
  42. X. Li, H. Liua, X. Niu et al., “The use of carbon nanotubes to induce osteogenic differentiation of human adipose-derived MSCs in vitro and ectopic bone formation in vivo,” Biomaterials, vol. 33, no. 19, pp. 4818–4827, 2012. View at Publisher · View at Google Scholar