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Journal of Nanomaterials
Volume 2012, Article ID 865186, 7 pages
http://dx.doi.org/10.1155/2012/865186
Research Article

Synthesis of Antibodies-Conjugated Fluorescent Dye-Doped Silica Nanoparticles for a Rapid Single Step Detection of Campylobacter jejuni in Live Poultry

1Risk and Decision Analysis Laboratory (RADAL), Food Safety Center, KMUTT and Department of Microbiology, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangkok 10140, Thailand
2Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
3Department of Physics, Faculty of Science, King Mongkut’s University of Technology, Thonburi, Bangkok 10140, Thailand
4Department of Science Services, Ministry of Science and Technology, Bangkok 10400, Thailand

Received 28 September 2011; Revised 28 December 2011; Accepted 29 January 2012

Academic Editor: Claude Estournes

Copyright © 2012 Wachira Tansub et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. EFSA, “The Community Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents, Antimicrobial Resistance and Foodborne Outbreaks in the European Union in 2006,” The EFSA Journal, 130 pages, 2007.
  2. WHO, “The Increasing Incidence of Human Campylobacteriosis. Report and Proceedings of a WHO Consultation of Experts,” WHO/CDS/CSR/APH/2000.4., 2000.
  3. D. Ivnitski, I. Abdel-Hamid, P. Atanasov, and E. Wilkins, “Biosensors for detection of pathogenic bacteria,” Biosensors and Bioelectronics, vol. 14, no. 7, pp. 599–624, 1999. View at Publisher · View at Google Scholar · View at Scopus
  4. D. Rodríguez-Lázaro, M. D'Agostino, A. Herrewegh, M. Pla, N. Cook, and J. Ikonomopoulos, “Real-time PCR-based methods for detection of Mycobacterium avium subsp. paratuberculosis in water and milk,” International Journal of Food Microbiology, vol. 101, no. 1, pp. 93–104, 2005. View at Google Scholar · View at Scopus
  5. B. W. Brooks, J. Devenish, C. L. Lutze-Wallace, D. Milnes, R. H. Robertson, and G. Berlie-Surujballi, “Evaluation of a monoclonal antibody-based enzyme-linked immunosorbent assay for detection of Campylobacter fetus in bovine preputial washing and vaginal mucus samples,” Veterinary Microbiology, vol. 103, no. 1-2, pp. 77–84, 2004. View at Publisher · View at Google Scholar · View at Scopus
  6. Y. Y. Wong, S. P. Ng, M. H. Ng, S. H. Si, S. Z. Yao, and Y. S. Fung, “Immunosensor for the differentiation and detection of Salmonella species based on a quartz crystal microbalance,” Biosensors and Bioelectronics, vol. 17, no. 8, pp. 676–684, 2002. View at Publisher · View at Google Scholar · View at Scopus
  7. S. Susmel, G. G. Guilbault, and C. K. O'Sullivan, “Demonstration of labeless detection of food pathogens using electrochemical redox probe and screen printed gold electrodes,” Biosensors and Bioelectronics, vol. 18, no. 7, pp. 881–889, 2003. View at Publisher · View at Google Scholar · View at Scopus
  8. S. Santra, P. Zhang, K. Wang, R. Tapec, and W. Tan, “Conjugation of biomolecules with luminophore-doped silica nanoparticles for photostable biomarkers,” Analytical Chemistry, vol. 73, no. 20, pp. 4988–4993, 2001. View at Publisher · View at Google Scholar · View at Scopus
  9. X. Zhao, R. Tapec-Dytioco, K. Wang, and W. Tan, “Conjugation of biomolecules with luminophore-doped silica nanoparticles for photostable biomarkers,” Analytical Chemistry, vol. 75, pp. 3476–3483, 2003. View at Google Scholar
  10. X. Zhao, R. Tapec-Dytioco, and W. Tan, “Ultrasensitive DNA detection using highly fluorescent bioconjugated nanoparticles,” Journal of the American Chemical Society, vol. 125, no. 38, pp. 11474–11475, 2003. View at Publisher · View at Google Scholar · View at Scopus
  11. R. P. Bagwe, C. Yang, L. R. Hilliard, and W. Tan, “Optimization of dye-doped silica nanoparticles prepared using a reverse microemulsion method,” Langmuir, vol. 20, no. 19, pp. 8336–8342, 2004. View at Publisher · View at Google Scholar · View at Scopus
  12. T. Suwanachart, Preparation of bioconjugated fluorescent-doped silica nanoparticles for the detection of Vibrio cholera O139, M.S. thesis, Applied Microbiology Program, Department of Microbiology, Faculty of Science King Mongkut's University of Technology Thonburi, Thailand, 2008.
  13. X. Hun and Z. Zhang, “Functionalized fluorescent core-shell nanoparticles used as a fluorescent labels in fluoroimmunoassay for IL-6,” Science Direct, vol. 22, pp. 1743–1748, 2007. View at Google Scholar
  14. R. P. Bagwe, L. R. Hilliard, and W. Tan, “Surface modification of silica nanoparticles to reduce aggregation and nonspecific binding,” Langmuir, vol. 22, no. 9, pp. 4357–4362, 2006. View at Publisher · View at Google Scholar · View at Scopus
  15. B. Du, Z. Li, and Y. Cheng, “Homogeneous immunoassay based on aggregation of antibody-functionalized gold nanoparticles coupled with light scattering detection,” Talanta, vol. 75, no. 4, pp. 959–964, 2008. View at Publisher · View at Google Scholar · View at Scopus