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Journal of Nanomaterials
Volume 2013, Article ID 194854, 9 pages
Research Article

Fabrication, Characterization, and Antimicrobial Activity, Evaluation of Low Silver Concentrations in Silver-Doped Hydroxyapatite Nanoparticles

1Faculty of Physics, University of Bucharest, 405 Atomistilor, P.O. Box MG-1, 077125 Magurele, Romania
2National Institute of Materials Physics, 105 Bis Atomistilor, P.O. Box MG 07, 077125 Magurele, Romania
3Microbiology Department, Faculty of Biology, University of Bucharest, Aleea Portocalelor 1-3, 60101 Bucharest, Romania

Received 14 February 2013; Accepted 18 April 2013

Academic Editor: Suad Rashdan

Copyright © 2013 A. Costescu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study was the evaluation of nanoparticles (Ag:HAp-NPs) for their antibacterial and antifungal activity. Resistance to antimicrobial agents by pathogenic bacteria has emerged in the recent years as a major public health problem worldwide. In this paper, we report a comparison of the antimicrobial activity of low concentrations silver-doped hydroxyapatite nanoparticles. The silver-doped nanocrystalline hydroxyapatite powder was synthesized at 100°C in deionised water. The as-prepared Ag:Hap nanoparticles were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), FT-IR, and FT-Raman spectroscopy. X-ray diffraction (XRD) studies demonstrate that powders obtained by coprecipitation at 100°C exhibit the apatite characteristics with good crystal structure, without any new phase or impurities found. FT-IR and FT-Raman spectroscopy revealed the presence of the various vibrational modes corresponding to phosphates and hydroxyl groups and the absence of any band characteristic to silver. The specific microbiological assays demonstrated that Ag:HAp-NPs exhibited antimicrobial features, but interacted differently with the Gram-positive, Gram-negative bacterial and fungal tested strains.