251063.fig.002a
(a)
251063.fig.002b
(b)
251063.fig.002c
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251063.fig.002d
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251063.fig.002e
(e)
Figure 2: Nanostructure-induced β-catenin translocation involves tyrosine phosphorylations. Confocal microscopy images of β-catenin distribution (red) in HMEC grown on the indicated substrate. Lower panels: representative profile plots along the indicated cell section (yellow line) show intensity distribution of β-catenin as relative immunofluorescence intensity ( -axis) along the distance in pixel ( -axis). Scale bars represent 10 μm. CTRL (a); for inhibitor experiments, cells were incubated for 6 h at 37°C with 50 nM PP2 (b), 3 μM dasatinib (DB, (c)), 2 μM imatinib (IB, (d)). Bar graphs representing the nuclear to cytosolic ratio of β-catenin immunofluorescence (e); values ± SEM; cells from 3 independent experiments, calculated from 3 different images of each experiment; ; compared to CTRL.