Research Article

β-Tricalcium Phosphate Micron Particles Enhance Calcification of Human Mesenchymal Stem Cells In Vitro

Figure 3

Effect of -TCP micron particles on proliferation and cytotoxicity of MSCs. (a) Scheme for assays in the contact condition and the noncontact condition. (b) Proliferation. MSCs were cultured with complete culture medium (CCM) supplemented with various concentrations of -TCP micron particles in the contact condition. Total cell number was measured by MTT assay. Averaged net absorbance with standard deviation is shown ( , ** by the Kruskal-Wallis test). (c) Morphology of total, living, and dead cells in the contact and noncontact condition. (d) The ratio of dead cells to total cells (%) with standard deviation (* by the Kruskal-Wallis test).
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