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Journal of Nanomaterials
Volume 2013, Article ID 453290, 6 pages
Research Article

Characterization and Stability Evaluation of Thymoquinone Nanoemulsions Prepared by High-Pressure Homogenization

1Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
2Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
3Department of Nutrition and Food Technology, Faculty of Agriculture, Hebron University, 90100 Hebron, Palestine
4Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia

Received 6 June 2013; Revised 25 September 2013; Accepted 25 September 2013

Academic Editor: Zhongkui Hong

Copyright © 2013 Zaki Tubesha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Despite the pharmacological properties of thymoquinone (TQ), its administration in vivo remains problematic partly due to its poor water solubility, leading to low absorptivity and bioavailability. Hence, the objective of this study is to prepare, characterize, and evaluate the stability of TQ nanoemulsion (TQNE). Conventional emulsion from TQ (TQCE) and empty nano- and conventional emulsions from Triolein (TRNE and TRCE) are also produced for comparison purposes. The oil-in-water nanoemulsions of TQ and Triolein were produced by high-pressure homogenization. Emulsions were characterized physically by droplet size, polydispersity index, zeta potential, and refractive index. The changes of these parameters in TQNE samples stored for 6 months at 4 and 25°C were not statistically significant ( ). In addition, the initial particle sizes of TQNE and TRNE were 119.6 and 119.5 nm, respectively. Stability studies were also performed for the period of 6 months. At the end of the experiment, the percent of remaining TQ in TQNE at 4, 25, and 40°C was 90.6, 89.1, and 87.4 % respectively. Slower degradation of TQ indicated the chemical stability of TQ in TQNE samples. These results indicated that TQNE is stable over a period of 6 months.