Bioaccumulation, Subacute Toxicity, and Tissue Distribution of Engineered Titanium Dioxide Nanoparticles in Goldfish (Carassius auratus)

Table 1

Expanded design summary of goldfish (Carassius auratus).

Parameter	Control	Group A	Group B
Volume of water in aquarium (L)	10	10	10
TiO2 NPs concentrations (mg/L)	0	10	100
Duration of exposure (day)	5	5	5
Water temperature (°C)	23±2	23±2	23±2
Oxygen (ppm)	6±1	6±1	6±1
pH value of water (Start–End)	6.30–6.05	6.03–6.15	6.63–6.45
Number of fish within the aquarium	5	5	5
Number of replications	2	2	2