A Novel Complex: A Quantum Dot Conjugated to an Active T7 RNA Polymerase

Figure 3

Activity test of purified QD : T7RNAP complexes. Lanes 1–3 and C1–C3 show the activity of 6 μL of the flow-through (F) and of the washed avidin resin (A). In lane 1 a total amount of 0.13 nmol Bio-T7 RNAP was used, in lane 2 0.091 nmol, and in lane 3 0.052 nmol. In all lanes 1–3 the amount of QDs was 0.01 nmol. C1, C2, and C3 are parallel controls of lanes l, 2, and 3, respectively, using the same amount of Bio-T7 RNAP but without QDs. Lane 4 shows the activity of 0.026 nmol free Bio-T7 RNAP, lane 5 1 pmol QDs alone, and lane 6 is a 100 bp DNA ladder. The red arrow marks the RNA product, green arrow marks DNA template, blue arrow marks QD : T7RNAP bands, and yellow arrow marks bands of free QDs. The material lighting up in the slots in the A lanes is the avidin resin unable to move into the gel, compared, for example, to lane 2A in Figure S3.