Synthesis of Silica-Coated Magnetic Nanoparticles and Application in the Detection of Pathogenic Viruses
Figure 7
Real-time PCR for qualitative measurement EBV virus load using DNA templates purified by Fe3O4/SiO2 nanoparticles and Dynabeads. (a) Amplification chart of EBV-74 fragments specific for EBV from sample 7, sample 10, standards, and positive and negative controls. : PCR product of positive control pGEM-EBV-74 at 5 × 108 copies/mL, negative controls, 103 to 108 copies/mL were labeled next to each respective amplification curves of standards. (b) Linear regression line based on standards and featured with samples 7 and 10 purified by Fe3O4/SiO2 nanoparticles and Dynabeads (). 1, 2, 3, and 4 were labeled next to amplification curves of samples number 7 by Nanoparticles, number 7 by Dynabeads, number 10 by Nanoparticles, and number 10 by Dynabeads, respectively.
