Melatonin Nanoparticles Adsorbed to Polyethylene Glycol Microspheres as Activators of Human Colostrum Macrophages

Table 2

Release of intracellular Ca²⁺ by colostrum phagocytes in the presence of melatonin adsorbed to PEG microspheres.

Colostrum mononuclear phagocytes (MN)	Fluorescence intensity; (mean ± SD)
PBS	13.96 ± 2.20
Cell + PEG	21.24 ± 1.26*
Cell + MLT	87.89 ± 8.38^*#
Cell + PEG + MLT	30.98 ± 5.03^*#

The intracellular Ca²⁺ release is represented by mean fluorescence intensity and was obtained by flow cytometry. The results represent the mean and SD of five experiments with cells from different individuals. ^∗𝑃<0.05: comparing the treated cells with non-treated cells (PBS); ^#𝑃<0.05: comparing the different treatments (MLT and PEG microspheres).