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Journal of Nanomaterials
Volume 2014, Article ID 148743, 13 pages
Research Article

Effect of CuO Nanoparticles over Isolated Bacterial Strains from Agricultural Soil

1Center for Research in Advanced Materials, Chihuahua, CHIH, Mexico
2Ingeniería Ambiental, Departamento de Ingeniería Civil, DI-CGT, Universidad de Guanajuato, Guanajuato, GTO, Mexico
3Campo Experimental Delicias, INIFAP, CHIH, Mexico
4Departamento de Astronomía, DCNyE-CGT, Universidad de Guanajuato, Guanajuato, GTO, Mexico
5Equipe Environment et Microbiologie, UMR IPREM5254, IBEAS, Université de Pau et des Pays de l’Adour, Pau, France

Received 26 June 2014; Accepted 4 December 2014; Published 31 December 2014

Academic Editor: Mallikarjuna N. Nadagouda

Copyright © 2014 Sandra I. Concha-Guerrero et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The increased use of the nanoparticles (NPs) on several processes is notorious. In contrast the ecotoxicological effects of NPs have been scarcely studied. The main current researches are related to the oxide metallic NPs. In the present work, fifty-six bacterial strains were isolated from soil, comprising 17 different OTUs distributed into 3 classes: Bacilli (36 strains), Flavobacteria (2 strains), and Gammaproteobacteria (18 strains). Copper oxide nanoparticles (CuONPs) were synthesized using a process of chemical precipitation. The obtained CuONPs have a spherical shape and primary size less than 17 nm. Twenty-one strains were used to evaluate the cytotoxicity of CuONPs and 11 of these strains showed high sensibility. Among those 11 strains, 4 (Brevibacillus laterosporus strain CSS8, Chryseobacterium indoltheticum strain CSA28, and Pantoea ananatis strains CSA34 and CSA35) were selected to determine the kind of damage produced. The CuONPs toxic effect was observed at expositions over 25 mg·L−1 and the damage to cell membrane above 160 mg·L−1. The electron microscopy showed the formation of cavities, holes, membrane degradation, blebs, cellular collapse, and lysis. These toxic effects may probably be due to the ions interaction, the oxide-reduction reactions, and the generation of reactive species.