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Journal of Nanomaterials
Volume 2014, Article ID 721267, 11 pages
Research Article

Optimization of Biohydrogen Production with Biomechatronics

1Department of Mechanical and Automation Engineering, Kao Yuan University, Kaohsiung 821, Taiwan
2Department of Applied Geoinformatics, Chia Nan University of Pharmacy & Science, Tainan 717, Taiwan

Received 10 February 2014; Accepted 19 February 2014; Published 26 March 2014

Academic Editor: Teen-Hang Meen

Copyright © 2014 Shao-Yi Hsia and Yu-Tuan Chou. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Massive utilization of petroleum and natural gas caused fossil fuel shortages. Consequently, a large amount of carbon dioxide and other pollutants are produced and induced environmental impact. Hydrogen is considered a clean and alternative energy source. It contains relatively high amount of energy compared with other fuels and by-product is water. In this study, the combination of ultrasonic mechanical and biological effects is utilized to increase biohydrogen production from dark fermentation bacteria. The hydrogen production is affected by many process conditions. For obtaining the optimal result, experimental design is planned using the Taguchi Method. Four controlling factors, the ultrasonic frequency, energy, exposure time, and starch concentration, are considered to calculate the highest hydrogen production by the Taguchi Method. Under the best operating conditions, the biohydrogen production efficiency of dark fermentation increases by 19.11%. Results have shown that the combination of ultrasound and biological reactors for dark fermentation hydrogen production outperforms the traditional biohydrogen production method. The ultrasonic mechanical effects in this research always own different significances on biohydrogen production.