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Journal of Nanomaterials
Volume 2014 (2014), Article ID 810404, 7 pages
http://dx.doi.org/10.1155/2014/810404
Research Article

Plasma Treated High-Density Polyethylene (HDPE) Medpor Implant Immobilized with rhBMP-2 for Improving the Bone Regeneration

1Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University, Gwangju 500-757, Republic of Korea
2Department of Oral Microbiology, School of Dentistry, Chonnam National University, Gwangju 500-757, Republic of Korea

Received 16 June 2014; Accepted 1 July 2014; Published 10 July 2014

Academic Editor: Seunghan Oh

Copyright © 2014 Jin-Su Lim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We investigate the bone generation capacity of recombinant human bone morphogenetic protein-2 (rhBMP-2) immobilized Medpor surface through acrylic acid plasma-polymerization. Plasma-polymerization was carried out at a 20 W at an acrylic acid flow rate of 7 sccm for 5 min. The plasma-polymerized Medpor surface showed hydrophilic properties and possessed a high density of carboxyl groups. The rhBMP-2 was immobilized with covalently attached carboxyl groups using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide. Carboxyl groups and rhBMP-2 immobilization on the Medpor surface were identified by Fourier transform infrared spectroscopy. The activity of Medpor with rhBMP-2 immobilized was examined using an alkaline phosphatase assay on MC3T3-E1 cultured Medpor. These results showed that the rhBMP-2 immobilized Medpor increased the level of MC3T3-E1 cell differentiation. These results demonstrated that plasma surface modification has the potential to immobilize rhBMP-2 on polymer implant such as Medpor and can be used for the binding of bioactive nanomolecules in bone tissue engineering.