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Journal of Nanomaterials
Volume 2014 (2014), Article ID 828141, 6 pages
http://dx.doi.org/10.1155/2014/828141
Research Article

Study on the Highly Sensitive AChE Electrode Based on Multiwalled Carbon Nanotubes

College of Science, University of Shanghai for Science and Technology, Shanghai 200093, China

Received 8 April 2014; Accepted 5 June 2014; Published 13 July 2014

Academic Editor: Sanqing Huang

Copyright © 2014 Shuping Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Using chitosan (CS) as carrier, the method named layer-by-layer (LBL) self-assembly modification to modify the glassy carbon electrode (GCE) with multiwalled carbon nanotubes (MWNTs) and acetylcholine esterase (AChE) was proposed to prepare the acetylcholine esterase electrode with high sensitivity and stability. The modified electrode was used to detect pesticide of aldicarb, and the enzyme inhibition rate of the electrode showed good linearity with pesticide concentrations in the range of 10−10 g·L−1 to 10−3 g·L−1. The detection limit was 10−11 g·L−1. The modified electrode was also used to detect the actual sample, and the recovery rate range was from 97.72% to 107.15%, which could meet the rapid testing need of the aldicarb residue. After being stored in the phosphate buffer solution (PBS) in 4°C for 30 days, the modified electrode showed good stability with the response current that was 80% of the original current.