Research Article | Open Access
C. Rodríguez-González, H. E. Cid-Luna, P. Salas, V. M. Castaño, "Hydroxyapatite-Functionalized Graphene: A New Hybrid Nanomaterial", Journal of Nanomaterials, vol. 2014, Article ID 940903, 7 pages, 2014. https://doi.org/10.1155/2014/940903
Hydroxyapatite-Functionalized Graphene: A New Hybrid Nanomaterial
Graphene oxide sheets (GO) were functionalized with hydroxyapatite nanoparticles (nHAp) through a simple and effective hydrothermal treatment and a novel physicochemical process. Microstructure and crystallinity were investigated by Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy, X-ray diffraction (XRD), ultraviolet-visible (UV-Vis) absorption spectroscopy, and thermogravimetric analysis (TGA). Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were performed to characterize the morphology of the functionalized material. The resulting novel materials combine the biocompatibility of the nHAp with the strength and physical properties of the graphene.
Graphene is a fascinating 2D nanomaterial whose importance has been recognized since it was isolated in 2004 . This carbon allotrope, which consists of a single layer of carbon atoms covalently bonded in a hexagonal network, exhibits exceptional properties such as high mechanical strength, optimal thermal conductivity, and excellent electrical conductivity [2–4]. Since basically all atoms in its surface are exposed, graphene presents an extremely large specific surface area (theoretical value of 2600 g/m2) making it an ideal candidate for the addition for various molecules [5, 6]. Moreover, graphene and graphene-related materials have shown potential biocompatibility, which is an essential issue if bioapplications, ranging from biomaterials to drug delivery systems, are to be considered [7–9].
Graphene oxide (GO) is a heavily oxygenated graphene derivative . Structurally, graphene oxide sheets consist of graphene sheets decorated with epoxy, hydroxyl, and ketone functional groups, above and below each plane, as well as carboxyl and carbonyl functionalities attached to the edges of the sheets. The abundance of functional groups on the surface of GO makes it highly hydrophilic . These functionalities can be used as chemical anchoring sites for different molecules, thus generating new composite materials with enhanced properties, as compared to their individual components [12–19].
Hydroxyapatite (HAp) is a compound made of calcium phosphate, the main crystalline component of the mineral phase of the bone . Given its chemical and structural similarity with the biological apatite, the synthetic hydroxyapatite possesses exceptional biocompatibility and lack of toxicity [21, 22]. In addition, HAp is osteoconductive; that is, it promotes bone-cells growth when it is placed in the vicinity of viable bone . As a result, HAp is being widely used in coatings on metal implants, as a filling for bone defects in orthopedic and dental applications, and also as reinforcement to polymer scaffold material for tissue regeneration .
Accordingly, it seems very attractive to develop a new class of hybrid material integrating mechanical properties and biological activity, which is highly promising to be used as a scaffold to promote the growth and differentiation of several classes of cells. It can also be used as a biocompatible phase reinforcement in biomedical composites and a drug delivery carrier , by synergetically combining GO and HAp. However, to date, there are few reports on methods for the fabrication of a GO-HAp nanocomposite. Neelgund et al., for instance, reported the functionalization of graphene sheets with hydroxyapatite through chemical precipitation . Liu et al. showed the simultaneous surface modification and reduction of GO sheets by polymerization of dopamine and then deposited the hydroxyapatite onto this surface by using a biomineralization method . Kim et al. synthesized graphene oxide-CaCO3 vaterite microspheres hybrid film, which enhanced hydroxyapatite formation when incubated in simulated body fluid solution . Liu et al. reported the synthesis of reduced graphene oxide and hydroxyapatite composite by chemical precipitation followed by spark plasma sintering consolidation .
Hydrothermal processing offers an interesting and effective route to synthesize nanocomposite materials using gentle reaction conditions. This approach produces highly crystalline and chemically homogeneous hydroxyapatite nanoparticles. Previous reports have also demonstrated it is convenient method for the reduction of graphene oxide sheets [28–30].
In this present study, we report the surface functionalization of graphene oxide sheets with hydroxyapatite through a low temperature hydrothermal synthesis. To the best of our knowledge, this synthesis method has not been reported yet. In our process, the influence of hydrothermal reaction time on the morphology of functionalized material was observed. The graphene-based hybrid material was morphological and microstructural characterized.
2. Materials and Methods
Pure crystalline graphite (300 meshes) was purchased from Electron Microscopy Science. Ammonium hydroxide (NH4OH) and diammonium hydrogen phosphate ((NH4)2HPO4) were obtained from Baker. All other reagents and solvents were obtained from Aldrich. All of the reagents were of analytical grade and used without any further purification.
2.2. GO Functionalization with HAp Nanoparticles
GO was synthesized by the modified Hummers method . A typical experiment, for the functionalization of GO with HAp nanoparticles, is as follows: 45 mg GO was dispersed in 15 mL DI water by ultrasonic treatment for 3 h. Then, 4.475 mL of Ca(NO3)2·4H2O (0.1 M) and 2.685 mL of ((NH4)2HPO4 (0.1 M) were added to the above system, obtaining the molar Ca/P ratio of 1.6, which corresponds to pure hydroxyapatite ratio. Furthermore, NH4OH was dropped in excess under constant stirring, adjusting the pH of the suspension to ≈10. The mixture was then transferred to a 70 mL teflon-lined autoclave and heated at 90°C under autogenous pressure for 6 and 24 hours. The hydrothermal synthesis of pure calcium hydroxyphosphate was based on the following reaction: The resulting suspension was naturally cooled to room temperature and washed with deionized water several times, in order to remove the NH4NO3. Finally, the sample was dried at room temperature for 24 h. In order to compare the obtained functionalized materials, pure HAp nanoparticles were also prepared by the same method.
The crystallinity and phase purity of the products were examined by powder X-ray diffraction (XDR) in a Rigaku Miniflex diffractometer with Cu Ka radiation ( nm). Fourier transform infrared (FTIR) spectra of KBr powder pressed pellets were recorded on a Bruker VECTOR 33 within the spectral region 400 to 4000 cm−1. Transmission electron microscopy (TEM) images were taken with a JEOL JEM1010 microscope. The morphology and elemental analysis of nanomaterial were carried out using a scanning electron microscopy (SEM) JEOL JSM-6060 LV equipped with an energy dispersive X-ray spectroscopy (EDX) spectrometer Oxford Inca X-Sight. Raman analysis was performed at room temperature using a LabRAM Dilor micro-Raman system equipped with an Argon ion laser with a wavelength of 488 nm. The absorption spectra of the samples were obtained using an ultraviolet visible spectroscopy (UV-Vis) recorded on a Hach spectrophotometer DR 5000. Thermal properties of GO and RGO/HA were measured by thermogravimetric analysis (TGA) on a SDT Q600 (TA Instruments) under a nitrogen atmosphere at a heating rate of 10°C/min.
3. Results and Discussion
FTIR was performed to verify GO reduction and functionalization with HAp nanoparticles, by the hydrothermal reaction. The characteristic absorption bands of GO (Figure 1(a)), including alkoxy C–O stretching (1030 cm−1), epoxy C–O stretching (1247 cm−1), O–H deformation vibrations of tertiary C–OH (1400 cm−1), and C=O stretching (1724 cm−1), were identified. Also, a strong and broad absorption at 3350 cm−1, due to O–H stretching vibrations, and an intense band at 1614 cm−1 corresponding to the C=C benzene ring mode was observed . As to the spectrum of prepared HAp (Figure 1(b)), the bands at 1035 and 1093 cm−1 correspond to the ν3 asymmetric stretching; 603 and 567 cm−1 are ν4 antisymmetric deformation . The peaks at 3570 and 631 cm−1 derive from the stretching and librational modes of the OH-ions, respectively . After hydrothermal reaction, both spectra of RGO/HAp 6 and 24 h (Figures 1(c) and 1(d)) showed that bands for oxygen functional groups of GO were significantly reduced and some of them disappeared entirely. Moreover, a new absorption band appeared at 1560 cm−1, which may be attributed to the C=C stretch vibration of graphene sheets . The absence of the O–H band at 631 cm−1, compared to Hap and the appearance of the C–O stretch band at 1192 cm−1, provided a solid indication of graphene oxide reduction and formation of the RGO/HAp composite. The spectrum of RGO/HAp at 24 h shows an increase in the width of the band at 1035 () and a decrease in the band at 3570 cm−1 (O–H), which could be attributed to an enhancement of the HAp particles on the GO surface.
Figure 2 shows the Raman spectra of GO (a) and HAp-graphene after 6 (b) and 24 (c) hours of hydrothermal reaction. For GO there are two characteristic peaks in the spectra, the band at 1590 cm−1 and the band at 1353 cm−1. The G band is associated with the mode of graphite, which is usually related to the in-phase vibrations of the graphite lattice, whereas the band is ascribed to the presence of disorder, vacancies, and edges in the network [35, 36]. In the Raman spectra of HAp-graphene 6 h and 24 h the band was shifted towards 1601 cm−1, while the intensity of the band decreased substantially. The shifting of the band may be due to the fact that the chemical interactions between carbon atoms of GO and reactive sites of HAp allow the formation of isolated double bonds, which resonate at higher frequencies . The disorder and graphitization degree of carbon materials may be quantified by analyzing the intensity ratio between and band . The ratios of GO, HAp-graphene 6 and 24 h were 1.03, 0.53, and 0.32, respectively, indicating that the functionalization of graphene with HAp greatly enhances the size of the in-plane domains of GO . In both HAp-graphene 6 and 24 h spectra the characteristic peaks for HAp at 447 cm−1 (ν2 ()), 554 cm−1 (ν4 ()), and 960 cm−1 (ν1 ()) [34, 39] were shown.
Figure 3 shows the XRD patterns of graphite (a), GO (b), and RGO/HAp 6 h (c) and 24 h (d). Graphite showed the characteristic (002) peak at around 2θ = 26.4°, corresponding to an average interlayer spacing of ~3.3 Å. The pattern of GO displayed the most intense peak at 2θ = 11.9° which corresponds to the (001) reflection. Due to the presence of oxygen-containing functional groups attached on both sides of the graphene sheet, GO has an average interlayer spacing of ~7.9 Å, indicating that individual graphene oxide sheets are thicker than pristine graphene sheets . The diffraction patterns of both RGO/HAp 6 and 24 h show characteristic peaks of the HAp hexagonal phase (JCPDS, card number 9-432). Well-defined peaks appeared at 2θ around 25.9°, 31.9°, 32.9°, 34.1°, 39.8°, 46.7°, and 50.5°, which are attributed, respectively, to the (002), (211), (300), (202), (310), (222), and (321) reflections of HA [41, 42]. The average particle size of HAp nanoparticles was calculated to be ca. 8.3 and 9.5 nm for RGO/Hap 6 and 24 h, respectively, based on Scherrer’s equation. No signal for any other phases about GO (001) can be detected in the RGO/HAp composite (Figure 3(c)). This may be due to the fact that GO can be reduced to graphene during the hydrothermal reaction, producing a restacking of the sheets forming poorly ordered graphitic structures; consequently, their diffraction peaks might also turn weak until disappearing . Moreover, because of functionalization, HAp nanoparticles act as spacers between graphene layers, which cannot be stacked to form detectable graphite structures; consequently, this weakens the diffraction of single carbon sheets .
RGO/HAp EDX spectrum (Figure 4) showed defined Ca, P, C, and O peaks that can be attributed to the presence of GO and HAp. Furthermore the Ca/P atomic ratio of the composite was 1.66, which is lower than the stoichiometric composition of pure hydroxyapatite but close to the calcium-deficient HAp, present in natural bone tissue .
The UV-Vis spectra for GO and graphene-based hybrids dispersions are shown in Figure 5. The spectrum obtained for GO dispersion displays a maximum absorption peak at 230 nm, which is characteristic of π-π* of aromatic C=C bonds, and a shoulder at about 300 nm attributed to transitions of C–O bonds . When the GO is functionalized with HAp nanoparticles, the peak at 230 nm is red shifted to 252 nm while the shoulder at 300 nm disappeared. This result suggests a strong interaction between graphene sheets and HAp nanoparticles and also the reduction of graphene oxide sheets [46, 47].
According to Figure 6(a), large and transparent graphene oxide sheets can be clearly observed in the TEM micrographs. The GO sheets exhibit wrinkled and crumpling morphologies illustrating a flake-like shape. The TEM images of RGO/HAp 6 h and RGO/HAp 24 h hybrid materials are shown in Figures 6(b) and 6(c), respectively. It is clearly seen in Figure 6(b) that rod-like HAp nanoparticles are well separated and distributed randomly onto the transparent graphene oxide sheets, indicating a strong interaction between graphene and nanoparticles [48, 49]. Figure 6(c) reveals graphene sheets uniformly decorated by the nanosized HAp particles. Also, the nanocomposite displays a high density of HAp nanoparticles. It is interesting to note that HAp nanoparticles are successfully dispersed onto graphene sheets in both hydrothermal reaction conditions. However, the density of HAp nanoparticles is higher in RGO/HAp 24 h nanocomposite because this hybrid material is subjected to a higher reaction time.
The SEM micrograph of Figure 7(a) displays layers of GO exhibiting a homogeneous surface with the characteristic wrinkles and ripples of graphene 2D structure. SEM images of RGO/HAp 6 h y RGO/HAp 24 h, Figures 7(b) and 7(c), respectively, show a completely different morphology as compared with the GO sheets. In both hybrid materials it can be observed that HAp nanoparticles are homogeneously deposited onto the graphene sheets. The surface of these nanocomposites shows a rough structure with many granules, indicating that GO sheets are covered by nHAp. These results are consistent with the results obtained by TEM, which showed a homogeneous distribution of HAp nanoparticles onto graphene oxide surface indicating that hydrothermal approach is an effective method to form graphene-based hybrid materials.
The TGA curve of GO (Figure 8(c)) showed an initial weight loss (12 wt%) before 100°C attributed to evaporation of water molecules contained in the material. The successive mass losses occurred below 250°C and above 550°C, which are attributed to the decomposition of labile oxygen-containing functional groups and the pyrolysis of the carbon skeleton, respectively . Compared with the GO weight loss at 250°C (40 wt%), the weight loss of RGO/HAp (Figure 8(b)) at 250°C is much lower (9 wt%), indicating a decreased amount of oxygenated functional groups after hydrothermal reduction. As for the TGA curve of HAp (Figure 8(a)), there is no significant weight loss from room temperature up to 1000°C, which is consistent with that reported in the literature . The curve of RGO/HAp (Figure 8(b)) showed that the sample had lost a significant mass (42 wt%) from 550 to 880°C, possibly due to the decomposition of graphene sheets. The residual weight measured for GO and RGO/HAp at 1000°C was found to be 4 wt% and 58 wt%, respectively, indicating the deposition of HAp nanoparticles over GO. Due to the thermal stability of HAp and after subtracting the water effect, it is estimated that about 61 wt% of HA is contained in the RGO/HAp composite.
Graphene oxide sheets were functionalized with hydroxyapatite nanoparticles through a hydrothermal reaction between Ca(NO3)2·4H2O and (NH4)2HPO4 in basic conditions at a temperature of 90°C. Both conditions of hydrothermal reaction time (6 h and 24 h) produce the deposition of hydroxyapatite nanoparticles free of impurities and other phases of calcium phosphates. However, a hydrothermal treatment time of 24 h produces graphene sheets highly decorated with nanohydroxyapatite with an average particle size (9.5 nm), larger in size compared to the ones obtained with a reaction time of 6 h. The results suggest that the hydrothermal process produced the functionalization and reduction of GO sheets to graphene, simultaneously.
Conflict of Interests
The authors declare that there is no conflict of interests regarding the publication of this paper.
The authors are grateful to Beatriz M. Millán-Malo for assistance with the XRD analysis, Genoveva Hernández-Padrón for FTIR analysis, Alicia del Real López for assistance with EDX and SEM, Ma. Lourdes Palma-Tirado for the TEM images, Damaris Cabrero-Palomino for TGA analysis, Francisco Rodríguez-Melgarejo for Raman analysis, and Ma. Cristina Piña-Barba for technical support. Claramaría Rodríguez-González is recipient of a postdoctoral fellowship from DGAPA/UNAM.
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