3D Nanoprinting Technologies for Tissue Engineering Applications

Figure 3

(a–c) Immunostaining of 10T1/2 cells for adhesion (nucleus, actin, and focal adhesions) and proliferation (EdU). (d) Schematic illustration showing cell in telophase: the cleavage furrow ingresses, compresses the midzone, and creates an intercellular bridge containing a microtubule midbody. In normal cell division, the bridge is resolved creating two daughter cells. The NPR structure induces aberrant cell-division response: (e–h) Abnormal cell division initiates and results in the formation of a long structure. Black arrow indicates persistent midbody connection. (i–m) Multiple sites of symmetric as well as asymmetric furrow formation during cell division on the NPR webs (dotted white arrows) (figures were reproduced from [30] with permission of John Wiley and Sons).

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