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Journal of Nanomaterials
Volume 2015, Article ID 234741, 10 pages
Research Article

Biosynthesis of Anisotropic Silver Nanoparticles by Bhargavaea indica and Their Synergistic Effect with Antibiotics against Pathogenic Microorganisms

1Department of Oriental Medicinal Biotechnology, College of Life Sciences, Kyung Hee University, Republic of Korea
2Graduate School of Biotechnology and Ginseng Bank, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea

Received 18 November 2014; Accepted 1 January 2015

Academic Editor: An-Ya Lo

Copyright © 2015 Priyanka Singh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The strain Bhargavaea indica DC1 isolated from four-year-old P. ginseng rhizospheric soil was used to perform rapid and extracellular biosynthesis of anisotropic silver nanoparticles. The ultraviolet-visible (UV-vis) spectra of the reaction mixture containing silver nanoparticles showed a peak at 460 nm, corresponding to the surface plasmon absorbance of silver nanoparticles. Field-emission transmission electron microscopy (FE-TEM) structural characterization revealed the nanobar, pentagon, spherical, icosahedron, hexagonal, truncated triangle, and triangular nanoparticles, with the size range from 30 to 100 nm. The energy-dispersive X-ray (EDX) analysis and elemental mapping results also confirmed that the silver was the predominant component of isolated nanoparticles. The X-ray diffraction (XRD) results correspond to the purity of silver nanoparticles and dynamic light scattering (DLS) result indicated that the average diameter of particles was 111.6 nm. In addition, enhancement in antimicrobial activity of commercial antibiotics was observed against various pathogenic microorganisms such as Vibrio parahaemolyticus, Salmonella enterica, Staphylococcus aureus, Bacillus anthracis, Bacillus cereus, Escherichia coli, and Candida albicans.