A Novel Approach of Targeted Immunotherapy against Adenocarcinoma Cells with Nanoparticles Modified by CD16 and MUC1 Aptamers

Figure 3

Evaluation of the conjugation of the aptamers with NP. MUC1 probe and CD16 probe were FAM-labeled DNA segments designed to hybridize with the MUC1 and the CD16 aptamers, respectively. Unmodified or aptamer-modified NPs were incubated with the probes and subjected to flow cytometry. Red lines represent signals from the beads treated with FAM-labeled DNA probe. Black lines represent the background signals from the untreated beads. (a) NP treated with MUC1 probe. (b) NP treated with CD16 probe. (c) CD16-NP treated with MUC1 probe. (d) CD16-NP treated with CD16 probe. (e) MUC1-NP treated with MUC1 probe. (f) MUC1-NP treated with CD16 probe. (g) CD16-NP-MUC1 treated with MUC1 probe. (h) CD16-NP-MUC1 treated with CD16 probe.

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