Frontal Cryosectioning: An Improved Protocol for Sectioning Large Areas of Fibrous Scaffolds

Figure 3

Construction of a three-dimensional model of cellular infiltration. (a) Images are imported into the workspace and separated on the -axis by a factor of 10. (b) A three-dimensional object of choice is used to replace each of the DAPI positive nuclei to represent cell location in space. (c) The bright field channel images depicting the scaffold fibers are turned off. (d) To better visualize cell penetration within the scaffolds a digital section was prepared and the opposing images were rotated to reveal the distribution of cells within the scaffold.

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