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Journal of Nanomaterials
Volume 2015 (2015), Article ID 562938, 12 pages
http://dx.doi.org/10.1155/2015/562938
Research Article

Dioscorea bulbifera Mediated Synthesis of Novel AucoreAgshell Nanoparticles with Potent Antibiofilm and Antileishmanial Activity

1Institute of Bioinformatics and Biotechnology, University of Pune, Pune 411007, India
2Nanomechanics Laboratory, Department of Physics, Indian Institute of Science Education and Research, Pune 411008, India
3Cell and Molecular Biology Laboratory, Department of Biotechnology, University of Pune, Pune 411007, India
4Department of Applied Physics, Defense Institute of Advanced Technology, Girinagar, Pune 411025, India
5Department of Chemical Engineering, Indian Institute of Technology, Bombay, Powai, Mumbai 400076, India
6Department of Microbiology, University of Pune, Pune 411007, India

Received 2 December 2014; Revised 12 March 2015; Accepted 19 March 2015

Academic Editor: Sherine Obare

Copyright © 2015 Sougata Ghosh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Dioscorea bulbifera is a potent medicinal plant used in both Indian and Chinese traditional medicine owing to its rich phytochemical diversity. Herein, we report the rapid synthesis of novel nanoparticles by D. bulbifera tuber extract (DBTE). NPs synthesis was completed within 5 h showing a prominent peak at 540 nm. HRTEM analysis revealed 9 nm inner core of elemental gold covered by a silver shell giving a total particle diameter upto 15 nm. NPs were comprised of % gold and % silver of the total mass. NPs showed highest biofilm inhibition upto % against A. baumannii. Biofilms of P. aeruginosa, E. coli, and S. aureus were inhibited up to %, %, and %, respectively. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) confirmed unregulated cellular efflux through pore formation leading to cell death. Potent antileishmanial activity of NPs (µg/mL) was confirmed by MTT assay. Further SEM micrographs showed pronounced deformity in the spindle shaped cellular morphology changing to spherical. This is the first report of synthesis, characterization, antibiofilm, and antileishmanial activity of NPs synthesized by D. bulbifera.