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Journal of Nanomaterials
Volume 2015 (2015), Article ID 564087, 9 pages
http://dx.doi.org/10.1155/2015/564087
Research Article

Preparation and Characterization of Nanofibrous Polymer Scaffolds for Cartilage Tissue Engineering

1Laryngology Department, Medical University of Silesia, 20 Francuska Street, 40-752 Katowice, Poland
2Faculty of Materials Science and Ceramics, AGH University of Science and Technology, 30 Mickiewicza Avenue, 30-059 Krakow, Poland
3Department of General, Molecular Biology and Genetics, Medical University of Silesia, 18 Medykow Street, 40-752 Katowice, Poland

Received 22 October 2014; Revised 19 December 2014; Accepted 19 December 2014

Academic Editor: Miguel A. Correa-Duarte

Copyright © 2015 Jarosław Markowski et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Polymer substrates obtained from poly(lactic acid) (PLA) nanofibres modified with carbon nanotubes (CNTs) and gelatin (GEL) for cartilage tissue engineering are studied. The work presents the results of physical, mechanical, and biological assessment. The hybrid structure of PLA and gelatine nanofibres, carbon nanotubes- (CNTs-) modified PLA nanofibres, and pure PLA-based nanofibres was manufactured in the form of fibrous membranes. The fibrous samples with different microstructures were obtained by electrospinning method. Microstructure, physical and mechanical properties of samples made from pure PLA nanofibres, CNTs-, and gelatin-modified PLA-nanofibres were studied. The scaffolds were also tested in vitro in cell culture of human chondrocytes collected from patients. To assess the influence of the nanofibrous scaffolds upon chondrocytes, tests for cytotoxicity and genotoxicity were performed. The work reveals that the nanofibrous structures studied were neither genotoxic nor cytotoxic, and their microstructure, physical and mechanical properties create promising scaffolds for potential use in cartilage repairing.