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Journal of Nanomaterials
Volume 2015, Article ID 568925, 7 pages
Research Article

Synthesis and Characterization of an Amphiphilic Linoleic Acid-g-Quaternary Chitosan with Low Toxicity

1State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau
2Translation Medicine Research Center, Lishui People’s Hospital, The Sixth Affiliated Hospital, Wenzhou Medical University, Lishui, Zhejiang 323000, China

Received 27 May 2015; Revised 20 July 2015; Accepted 26 July 2015

Academic Editor: Jin-Ho Choy

Copyright © 2015 Xiaobin Fang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A novel amphiphilic derivative of chitosan, namely, a linoleic acid-g-quaternary chitosan (LA-g-QC), was designed and synthesized as low toxic material for biomedical applications in this study. The chemical structure of LA-g-QC was characterized by Fourier transform infrared spectroscopy (FTIR), 1H nuclear magnetic resonance (1H-NMR), and elemental analysis. LA-g-QC could form nanosized micelles with self-assembly, which was confirmed by the results of critical micelle concentration (CMC) via fluorescence spectroscopy. The average size of LA-g-QC was 140 nm and its zeta potential was approximately +35.50 mV. CMC value was 31.00 mg/mL. Furthermore, LA-g-QC micelles, at final concentrations between 0.94 μg/mL and 30 μg/mL, did not inhibit the proliferation of HepG2 or SMMC 7721 cell lines. Taken together, LA-g-QC has low cytotoxicity and high potential for the preparation of novel drug-delivery micelles.