Table of Contents Author Guidelines Submit a Manuscript
Journal of Nanomaterials
Volume 2015, Article ID 616879, 6 pages
Research Article

Horseradish Peroxidase-Carrying Electrospun Nonwoven Fabrics for the Treatment of o-Methoxyphenol

1College of Science, Dalian Ocean University, Dalian, Liaoning 116023, China
2College of Science, Dalian Nationalities University, Dalian 116600, China

Received 3 July 2014; Revised 7 September 2014; Accepted 21 September 2014

Academic Editor: Yuqin Wan

Copyright © 2015 Chao Pan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The carboxyl-functionalized polystyrene (poly(styrene-co-methacrylic acid), PSMAA) nanofibers with average diameters of 250 ± 20 nm was prepared by electrospinning. PSMAA nanofibrous membrane were employed for immobilization of horseradish peroxidase (HRP) enzyme on the fibrous surface by a chemical method. The parameters about immobilizing HRP on the PSMAA nanofibers were studied and the influence on the activity of the HRP is discussed. This study showed that soap-free emulsion method is an ideal technology to modify the polystyrene surface and ultimately achieve enzyme immobilization on electrospun PSMAA nanofibers surfaces. Compared with free HRP, the acid-base stability, thermal stability, and storage stability of HRP were increased after the immobilization. The immobilized HRP maintained about 60% of its initial activity during a 20-day storage period. However, the free HRP maintained only 40% of its initial activity. The removal percentages of o-methoxyphenol (OMP) reached 80.2% after 120 min for immobilized HRP. These results suggest that the proposed scheme for immobilization of HRP has potential in industrial applications for the treatment of phenolic wastewater.