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Journal of Nanomaterials
Volume 2015, Article ID 674175, 12 pages
http://dx.doi.org/10.1155/2015/674175
Research Article

Optimization and Characterization of Artesunate-Loaded Chitosan-Decorated Poly(D,L-lactide-co-glycolide) Acid Nanoparticles

1National Institute of Pharmaceutical Technology, Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hoan Kiem, Ha Noi 100000, Vietnam
2Hue College of Medicine and Pharmacy, Hue University, 6 Ngo Quyen, Hue, Thua Thien Hue 530000, Vietnam
3College of Pharmacy, Yeungnam University, 214-1 Dae-Dong, Gyeongsan 712-749, Republic of Korea

Received 4 September 2015; Accepted 3 November 2015

Academic Editor: Ilaria Armentano

Copyright © 2015 Hoang Nhan Ho et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to optimize the formulation of artesunate-loaded chitosan- (CS-) decorated poly(D,L-lactide-co-glycolide) acid (PLGA) nanoparticles as well as evaluate their characteristics. CS-to-PLGA mass ratio, pH of CS solution, and experimental temperature were optimized using response surface methodology to understand their effects on size and zeta potential of nanoparticles. The optimized formulation showed the close agreement between predicted and experimental values (all bias below 5%). The presence of CS was confirmed by positive surface charge and Fourier transform infrared spectroscopy. A spherical-like shape of particles was observed in range of small size around 190 nm. This CS layer restricted initial burst release of drug from carriers in phosphate buffer of pH 6.8. In addition, CS-coated NPs enhanced the intracellular uptake, in vitro cytotoxicity, and apoptosis-induced nuclei behaviors compared with CS-uncoated NPs as well as free drug in MCF-7 and A549 cancer cells.