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Journal of Nanomaterials
Volume 2015, Article ID 704817, 7 pages
Research Article

Targeted In Vivo Imaging of Mouse Hindlimb Ischemia Using Fluorescent Gelatin Nanoparticles

1State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China
2Tianjin Union Medicine Center (Nankai University Affiliated Hospital), Clinical and Translation Medicine Lab, Tianjin 300121, China

Received 16 October 2014; Accepted 17 December 2014

Academic Editor: Shuming Zhang

Copyright © 2015 Ju Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Critical limb ischemia is one of the most advanced forms of peripheral artery disease, which seriously threat the human health and even cause amputation. In this study, we developed a fluorescent gelatin nanoparticle (FGNP) by covalent conjugation of the nanoparticles with two fluorogens, Cy7 and rhodamine B. The FGNPs have a volume average hydrodynamic diameter of about 168 nm, which also show low cytotoxicity against NIH/3T3 normal cells. The in vivo ischemia bioimaging studies in live mice and in ischemic limb slices demonstrate that the FGNPs can be preferentially accumulated to the ischemic site, which can thus serve as a safe and effective probe for targeted visualization of ischemia in the limb.