Surface Epitope Coverage Affects Binding Characteristics of Bisphenol-A Functionalized Nanoparticles in a Competitive Inhibition Assay

Figure 3

Dose-response curves of the ci-ELISA under the same epitope coverage. The absorbance curve (a) and inhibition curve using the molar concentration of cysBPAv-AuNP in an assay (b). ● = 6.6 × 10⁻¹⁰ mol L⁻¹ cysBPAv-AuNP-265 (molar concentration of epitope in an assay: 1.70 × 10⁻⁷ mol L⁻¹); □ = 3.3 × 10⁻¹⁰ mol L⁻¹ cysBPAv-AuNP-265 (molar concentration of epitope in an assay: 9.00 × 10⁻⁸ mol L⁻¹); ▲ = 1.3 × 10⁻¹⁰ mol cysBPAv-AuNP-265 L⁻¹ (molar concentration of epitope in an assay: 4.00 × 10⁻⁸ mol L⁻¹).

(a)

(b)