Surface Epitope Coverage Affects Binding Characteristics of Bisphenol-A Functionalized Nanoparticles in a Competitive Inhibition Assay

Table 4

Assay parameters and calculated affinity constant derived from Figure 5.

Nanoparticle	190 (●)	396 (□)	801 (▲)
Particle number (mol L^−1)	5.3 × 10^−10	2.6 × 10^−10	1.3 × 10^−10
Molar concentration of surface epitope (mol L^−1)	1.0 × 10^−7	1.0 × 10^−7	1.0 × 10^−7
Slope from Figure 5(b)	0.65 ± 0.11	0.62 ± 0.07	1.0 ± 0.13
IC50 (µmol L^−1)	3.6 ± 1.4	4.7 ± 1.6	2.8 ± 1.3
Affinity constant (, µmol^−1)	0.30 ± 0.05	0.27 ± 0.04	0.33 ± 0.05
Linear detection range (µmol L^−1)	0.47–24.6	0.74–40.7	0.52–43.7

20–80% of the % curve.