Controlling Surface-Induced Nanocomposites by Lipoplexes for Enhanced Gene Transfer

Figure 3

(a) SEM images of surface-induced nanocomposites formed from the indicated mineralizing solutions. The scale bar is 200 nm. (b) AFM topographic images (top 3 panels) and 3D surface plot (bottom 3 panels) of surface-induced nanocomposites on substrates formed from the indicated formulations. The scan size is 2 μm × 2 μm. (c) Surface roughness (, root mean square roughness) of surface-induced nanocomposites on substrates formed from the indicated mineralizing solution. was calculated using NanoScope (Veeco, Plainview, NY). Data were shown as means ± s.e. Statistical significance of differences was determined by the unpaired two-tailed Student’s -test. The of substrates deposited with lipoplex-nanocomposites was considered statistically significantly different from that with DNA-nanocomposites at . (d) The surface plot of the fluorescence intensity of FITC-labeled DNA on the surfaces deposited with DNA- or lipoplex-nanocomposites. (e) The profiles of fluorescent intensity along the diagonal direction determined using ImageJ (National Institute of Health) was used to demonstrate the DNA distribution.

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