Development of Bioactive Patch for Maintenance of Implanted Cells at the Myocardial Infarcted Site

Figure 5

General view of the PEA and PCLMA bioactive implants on mice heart after 3 days. (a) Whole heart excision and macroscopic examination. ((A) and (B)) Macroscopic view of mouse heart and bioactive implant (outlined) at 3 days after implantation, and ((C) and (D)) Masson’s trichrome staining of heart with bioactive implant cross section. The dotted zone in black corresponds to the bioactive implant and the dotted red area to the infarcted zone. (b) Microscopic view of the bioactive implant. ((E) and (H)) Detail of elongated subATDPCs (red) inside the PEA (E) and PCLMA (H) bioactive implants; ((F) and (I)) views of the PEA (F) and PCLMA (I) bioactive implants attached to the myocardium (implant and myocardium are limited by dotted lines (Phalloidin staining in red)); and ((G) and (J)) migration of the subATDPCs (white arrows) to the myocardium (cTnI staining in white) in PEA (G) and PCLMA (J) groups. Constitutive expression of RFP (red) in subATDPCs. Nuclei were counterstained with Hoescht 33342 (blue). Scale bars 1 mm ((A)–(D)), 50 μm ((E)–(H) and (J)), and 100 μm (I).

(a)

(b)