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Journal of Nanomaterials
Volume 2015 (2015), Article ID 896185, 6 pages
Research Article

Laser-Assisted Synthesis of Mn0.50Zn0.50Fe2O4 Nanomaterial: Characterization and In Vitro Inhibition Activity towards Bacillus subtilis Biofilm

1Department of Physics, University of Agriculture, Faisalabad 38040, Pakistan
2Department of Chemistry, University of Sargodha, Sargodha 40100, Pakistan
3Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia
4Department of Biochemistry, University of Agriculture, Faisalabad 38040, Pakistan
5Department of Applied Chemistry & Biochemistry, Government College University, Faisalabad 38000, Pakistan
6Deanship of Scientific Research College of Engineering, King Saud University, P.O. Box 800, Riyadh, Saudi Arabia

Received 19 November 2014; Accepted 16 February 2015

Academic Editor: Ali Khorsand Zak

Copyright © 2015 Shaukat Ali Shahid et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


There is growing interest in the development of novel nanomaterials with potential antimicrobial activity and lesser toxicity. In the current research work, Mn0.5Zn0.5Fe2O4 nanoparticles were synthesized via a novel coprecipitation cum laser ablation technique yielding fine spinal structured material. The synthesized nanomaterial was structurally characterized by X-ray diffraction technique which confirmed the formation and the crystalline nature of Mn0.50Zn0.50Fe2O4 nanomaterial. The crystallite size determined by Debye-Scherrer’s formula was found to be ~12 nm. The formation of nanoparticles was evidenced by scanning electron microscopy. Energy dispersive X-ray analysis (EDXA) was performed for elemental analysis. The synthesized nanomaterial was interestingly found to be an effective antimicrobial agent and inhibited the growth of Bacillus subtilis biofilm formation. The 5 µg of Mn0.5Zn0.5Fe2O4 nanomaterial dissolved in 1 mL of DMSO showed excellent biofilm inhibitory activity 91.23% ± 1.87 against Bacillus subtilis.