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Journal of Nanomaterials
Volume 2015, Article ID 920893, 9 pages
Research Article

Comparison of Fibroblast and Osteoblast Response to Cultivation on Titanium Implants with Different Grain Sizes

1Department of Medical Chemistry and Biochemistry, Faculty of Medicine in Pilsen, Charles University in Prague, Karlovarska 48, 301 66 Pilsen, Czech Republic
2Department of Biology, Faculty of Medicine in Pilsen, Charles University in Prague, Alej Svobody 76, 323 00 Plzen, Czech Republic
3Biomedical Center, Faculty of Medicine in Pilsen, Charles University in Prague, Alej Svobody 76, 323 00 Pilsen, Czech Republic
4Department of Applied Electronics and Telecommunications, Faculty of Electrical Engineering, University of West Bohemia, Univerzitni 26, 306 14 Pilsen, Czech Republic
5Department of Stomatology, Faculty of Medicine in Pilsen, University Hospital and Charles University, Alej Svobody 80, 301 00 Pilsen, Czech Republic

Received 24 September 2015; Revised 23 November 2015; Accepted 26 November 2015

Academic Editor: Daniel S. Oh

Copyright © 2015 Vaclav Babuska et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The in vitro response of human fibroblast cell line HFL1 and human osteoblast cell line hFOB 1.19 on nanostructured titanium with different grain sizes has been compared in the present study. Used samples of titanium produced by equal channel angular (ECA) pressing have grain sizes of 160 nm, 280 nm, and 2400 nm with cross- and longitudinal sections. Similar cellular behaviour was observed on all studied biomaterials. There were significant differences related to the initial phase of attachment, but not in proliferation. Furthermore, the results indicate that osteoblasts grow best on material with grain size of 160 nm with a longitudinal section in comparison with other examined materials. Therefore, this material could be recommended for further evaluation with respect to osseointegration in vivo.