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Journal of Nanomaterials
Volume 2016, Article ID 2368630, 10 pages
Research Article

Silver Nanoparticles Embedded in Natural Rubber Films: Synthesis, Characterization, and Evaluation of In Vitro Toxicity

1Departamento de Física, FCT-UNESP, 19060-900 Presidente Prudente, SP, Brazil
2Agência Paulista de Tecnologia de Agronegócios, Polo Regional Alta Sorocabana, 19160-000 Presidente Prudente, SP, Brazil
3Universidade do Oeste Paulista, 19050-920 Presidente Prudente, SP, Brazil
4Department of Chemistry and Biochemistry, University of Windsor, Windsor, ON, Canada N9B 3P4
5Cellular Materials Laboratory (CellMat), Condensed Matter Physics Department, University of Valladolid, 47011 Valladolid, Spain

Received 20 April 2016; Accepted 16 June 2016

Academic Editor: Xuping Sun

Copyright © 2016 Caroline S. Danna et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Natural rubber (NR) films can reduce silver metal ions forming embedded metal nanoparticles, a process that could be described as green synthesis. The NR films acting as a reactor generate and incorporate silver nanoparticles (AgNPs). Organic acids and amino acids play a crucial role in the formation of AgNPs. The plasmon extinction obtained in the UV-visible spectrum shows the presence of nanoparticles in the film after dipping the NR film into a solution of silver nitrate at 80°C. Electron microscopic analysis confirms the presence of AgNPs in the NR film and characterization by atomic force microscopy shows a change in the roughness of the NR film with AgNPs. In addition, our preliminary results from in vitro toxicity studies (MTT and comet assays) of the NR films and NR films with silver nanoparticles (NR/Ag) show that they are not toxic to cell lineage CHO-K1 (cells from the ovary of a Chinese hamster), an important result for potential medical applications.