Delivery Efficiency of miR-21i-CPP-SWCNT and Its Inhibitory Effect on Fibrosis of the Renal Mesangial Cells

Figure 4

The CLSM analysis of TGF-β1 and α-SMA proteins in RMCs after being treated with miR-21is and their different hybrids (miR-21i-CPP, miR-21i-SWCNT, and miR-21i-CPP-SWCNT) for 24 hrs. (a–e) The CLSM analysis of TGF-β1 proteins in different groups. (blue) Cell nuclei marked by DAPI, (red) TGF-β1 positive expression, and (green) FAM-labeled miR-21i fragments. (f–j) The CLSM analysis of α-SMA proteins in different groups. (blue) Cell nuclei marked by DAPI, (red) α-SMA positive expression, and (green) FAM-labeled miR-21i fragments. (a and f) double distilled PBS; (b and g) miR-21i alone; (c and h) miR-21i-CPP hybrids; (d and i) miR-21i-SWCNT hybrids; (e and j) miR-21i-CPP-SWCNT. Scale bar = 10 μm.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)