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Journal of Nanomaterials
Volume 2016, Article ID 3948768, 10 pages
Research Article

In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA

1Institute of Nuclear Sciences “Vinča”, University of Belgrade, Mike Petrovića Alasa 12-14, 11001 Belgrade, Serbia
2Department of Pediatric and Preventive Dentistry, Faculty of Dentistry, University of Belgrade, Dr. Subotića 8, 11000 Belgrade, Serbia
3Clinic for Maxillofacial Surgery, Faculty of Dentistry, University of Belgrade, Dr. Subotića 4, 11000 Belgrade, Serbia
4Faculty of Medicine, University of Niš, Bulevar Dr. Zorana Đinđića 81, 18000 Niš, Serbia
5Institute for Surgery, Orthopedics and Ophthalmology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobođenja 18, 11000 Belgrade, Serbia

Received 6 December 2015; Revised 16 March 2016; Accepted 22 March 2016

Academic Editor: Ilaria Armentano

Copyright © 2016 Vukoman Jokanović et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A synthetic bone substitute based on calcium hydroxyapatite (CHA) and poly(lactic-co-glycolic) acid (PLGA), described in this paper, was synthesized to fulfill specific requirements like biodegradability, satisfying mechanical properties, optimal porosity and nanotopology, osteoinductive and osteoconductive properties, and so forth. Structural and morphological properties of the new scaffold were analyzed by micro computed tomography and scanning electron microscopy, while its physicochemical properties were investigated by X-ray diffraction and infrared spectroscopy. In vivo biological investigations of the synthesized scaffold were conducted over the cutaneous irritation and biofunctionality assays on rabbits and the test of acute systemic toxicity on mice. The results showed that the scaffold is not irritant and that it does not exhibit any symptoms of acute toxicity. Biofunctionality assays which include evaluation of the presence of various cells of immune response, the presence of neoangiogenesis, percentage of mineralization of newly formed bone, and fibroplasia in the tissue indicated that the new scaffold is suitable for the application in maxillofacial and dental surgery as a bone substitute. Also, it showed significant advantages over commercial product Geistlich Bio-Oss® from the aspect of some parameters of immunological response.