Research Article
Redox-Responsive Nanophotosensitizer Composed of Chlorin e6-Conjugated Dextran for Photodynamic Treatment of Colon Cancer Cells
Figure 6
(a) ROS generation and (b) phototoxicity of DEX6ss nanoparticles. For ROS generation measurement, HCT116 cells were incubated with various concentration of Ce6 or DEX6ss nanoparticles for 2 h with DCFH-DA. Cell were washed with PBS and then 100 μL phenol red-free RPMI media was added to each well following irradiation at 2.0 J/cm2 light. ROS generation was measured based on fluorescence intensity and expressed as the percentage versus control. For phototoxicity measurement, cells were incubated with various concentrations of Ce6 or DEX6ss nanoparticles for 2 h and washed with PBS. Then, fresh media were added and irradiated with 2.0 J/cm2 light. The presented data was mean ± s.d. from 8 wells of 96-well plates.
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