DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts
Gene expression of the mCherry in Candida. (a)–(c) Control: Candida cells without any exposure to DNA microspheres. (d)–(f) Candida cell after incubation with DNA microspheres encoding to mCherry expression, coated with PCL. (g) Fluorescence calculations of the mCherry expression inside the cells. Red fluorescence (a, d) (rhodamine mode: = 587 nm, = 610 nm); (b, e) bright-field images (DIC); the merged figures (c, f) represent the data obtained when two filters are used.