DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts
Gene expression of the GFP in Candida. (a)–(c) Control: Candida cells without any exposure to DNA microspheres. (d)–(f) Candida cell after incubation with DNA microspheres encoding to GFP expression, coated with PCL. (g) Fluorescence calculations of the GFP expression inside the cells. (a, d) Green fluorescence mode ( 488 nm, 507 nm filter = green); (b, e) bright-field images (DIC); the merged figures (c, f) represent the data obtained when two filters are used.