Studies on the Genotoxicity Behavior of Silver Nanoparticles in the Presence of Heavy Metal Cadmium Chloride in Mice

Table 2

Sperm concentrations, sperm motility, and abnormal sperms frequency in mice injected i.p. with Ag nanoparticles or/and CaCl₂.

Group	Treatment; (dose mg/kg)	Sperm concentration; (10^6/mL)	Sperm motility (%)	Abnormal sperms (%)
1	Negative control	1.44 ± 0.04	77.04 ± 1.68	5.20 ± 1.30
2	Cd(1.6)	0.58 ± 0.15	29.63 ± 3.26	58.20 ± 4.66
3	Ag(20)	1.18 ± 0.06	55.09 ± 3.55	18.40 ± 3.21
4	Ag(41)	0.92 ± 0.07	30.44 ± 2.48	37.6 ± 2.30
5	Ag(82)	0.45 ± 0.05	21.92 ± 2.05	59.00 ± 2.24
6	Ag(20) + Cd	0.98 ± 0.05	35.47 ± 2.63	31.20 ± 2.38
7	Ag(41) + Cd	0.70 ± 0.08	23.86 ± 2.96	52.20 ± 2.59
8	Ag(82) + Cd	0.36 ± 0.04	10.71 ± 1.72	74.60 ± 4.56
Groups 3, 4, and 5		= 194, < 0.001	= 193, < 0.001	= 300, < 0.001
Groups 6, 7, and 8		= 137, < 0.001	= 124, < 0.001	= 216, < 0.001

Results were expressed as mean ± SD. Statistically significantly different from the negative control group at < 0.001 and statistically significantly different from the comparable nano-Ag treated group at < 0.01 and < 0.001, respectively, using Student’s -test. One way ANOVA was used to test the effect of different doses of Ag nanoparticles on the tested parameters.