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Journal of Nanomaterials
Volume 2016, Article ID 5432656, 9 pages
Research Article

SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System

1Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (CONICET-UNLP), CC16 Suc4, No. 1900, La Plata, Buenos Aires, Argentina
2Instituto de Estudios Inmunológicos y Fisiopatológicos (CONICET-UNLP), La Plata, Argentina
3Centro Atómico Bariloche, Instituto Balseiro, CNEA, 8400 S. C. de Bariloche, Rio Negro, Argentina
4EEA Anguil (INTA), Ruta Nac. No. 5 km 580, CC 11 (6326), Anguil, La Pampa, Argentina

Received 24 June 2016; Revised 4 October 2016; Accepted 19 October 2016

Academic Editor: Felix J. Seo

Copyright © 2016 M. Antonieta Daza Millone et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The Supporting Information contains UV-vis spectra of BSA, BSA-DNP and DNBS reactive employed in conjugation in PBS buffer solution; MALDI-TOF spectra of BSA and BSA-DNP conjugate; SPR curves fitting with Winspall at 670 nm and 785 nm; Illustration of the different steps involved in the construction of the Au-MUA-PLL-DNP platform; SPR binding isotherms of BSA-DNP covalently linked to MUA monolayer and anti-DNP in solution and SPR binding isotherms of BSA-DNP covalently linked to MUA or to mixed MUA/DTT monolayers and soluble anti-DNP.

  1. Supplementary Material