Gold Nanoparticles Synthesized with a Polyphenols-Rich Extract from Cornelian Cherry (Cornus mas) Fruits: Effects on Human Skin Cells

Figure 9

Inflammatory cytokine levels in HaCaT cells’ culture medium following treatment with GNPs-CM (6 μg/mL). (a) IL-1α secretion by HaCaT cells treated with GNPs-CM (dark) shows no modification in this cytokines’ secretion at neither time point; the treatment with gold NPs before UVB irradiation has no significant influence on IL-1α synthesis. The presence of gold NPs has no influence on IL-1α synthesis after irradiation; (b) TNF-α levels after 6, 12, and 24 h incubation with GNPs-CM show no differences compared to control cells (dark). Irradiation only (UVB) results in significantly higher TNF-α levels at the 12 h time point, while the presence of GNPs-CM reduces TNF-α secretion to the levels found in nonirradiated cells; (c) IL-6 secretion is not affected by GNPs-CM (dark); UVB exposure increases IL6 release, mostly at 12 h after exposure; GNPs-CM treatment increases IL-6 levels in irradiated cells, mostly at 24 h.

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