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Journal of Nanomaterials
Volume 2016, Article ID 7135852, 15 pages
Research Article

Enhancement of the Antibacterial Activity of Silver Nanoparticles against Phytopathogenic Bacterium Ralstonia solanacearum by Stabilization

Laboratory of Natural Product Pesticide, College of Plant Protection, Southwest University, Chongqing 400715, China

Received 16 December 2015; Revised 5 April 2016; Accepted 5 May 2016

Academic Editor: Piersandro Pallavicini

Copyright © 2016 Juanni Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In this paper, the enhanced antibacterial activity of silver nanoparticles (AgNPs) against the phytopathogenic bacterium Ralstonia solanacearum after stabilization using selected surfactants (SDS, SDBS, TX-100, and Tween 80) was examined, in comparison with silver ion. Tween 80 was found to be the most preferable stabilizer of AgNPs due to the beneficial synergistic effects of the AgNPs and surfactant. However, all the surfactants nearly had no effects on the antibacterial activity of Ag+. In vitro, Tween 80-stabilized AgNPs showed the highest bactericidal activity against R. solanacearum. Further measurements using TEM, fluorescence microscopy, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that though Ag+ and Tween 80-Ag+ induced high toxicity, Tween 80-stabilized AgNPs displayed most severe damage when in direct contact with cells, causing mechanistic injury to the cell membrane and strongly modifying and destructing the cellular proteins. Meanwhile, in vivo, the pot experiments data indicated that the control efficiency of Tween 80-stabilized AgNPs on tobacco bacterial wilt was 96.71%, 90.11%, and 84.21%, at 7 days, 14 days, and 21 days, respectively. Based on the results evidencing their advantageous low dosage requirements and strong antimicrobial activity, Tween 80-stabilized AgNPs are a promising antibacterial agent for use in alternative crop disease control approaches.