The synthesis of gold nanoparticles (Au-NPs), using Pluronic P103 as soft template to design tuned hybrid gold/P103 nanomaterials, is reported here. The effect of the concentration of P103 and the synthesis temperature on the growth, size, and morphology of Au-NPs were studied. The rheological properties of these hybrid nanomaterials at different measured temperatures were studied as well. By increasing the concentration of P103, the micelles progressively grew due to an increase in the number of surface cavities. These cavities came together causing large nucleation centers and developing larger Au-NPs. The synthesis temperature was varied to induce significant dehydration of the P103 micelles. Below the cloud point temperature micelles underwent distinct changes related to spherical-to-polymer-like micelles transitions. Two nanostructures were formed: (1) small Au-NPs arranged on the surface of micelles, which acted as soft templates, and (2) large and independent Au-NPs. Above the cloud point temperature, Au-NPs were related to the shape and size of the P103 micellar aggregates. Rheological measurements showed that viscosity was sensitive to the concentration of P103. Also, it was demonstrated that synthesis temperature had a considerable influence on viscosity of the produced nanomaterials.

1. Introduction

Hybrid nanomaterials fabricated from polymers and inorganic nanoparticles (NPs) have been the subject of extensive investigations due to their distinct advantages, such as improved water solubility, stimuli-responsiveness, excellent biocompatibility, and facile introduction of functional units. Therefore, promising applications in the field of imaging, detection, drug/gene delivery, diagnostics, and nanoreactors are being extensively investigated [1]. Polymer assemblies provide excellent templates or matrices for the spatial organization of inorganic NPs, resulting in hybrid nanomaterials [2]. Sakai and Alexandridis [3] have reported a single-step synthesis of gold nanoparticles (Au-NPs) using poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymers, commercially known as Pluronic.

The Pluronic types are capable of forming micelles in aqueous media due to their amphiphilic character [4]. At low Pluronic concentrations, spherical micelles grow progressively to form polymer-like micelles as the temperature is increased. In this sense, the temperature at which micelles are formed is known as the critical micelle temperature (CMT). Heating beyond the CMT drastically reduces the hydration of micelles, resulting in a predominantly separated hydrophobic phase. The temperature where the phase separation occurs is known as the cloud point temperature (CPT) [58].

Micelles formed by Pluronic P103 have surface cavities that are in direct contact with the chloroauric acid aqueous solution (); thus they are mainly responsible for the reduction of gold ions to form gold nanoparticles [2]. For instance, by varying the copolymer concentration and temperature, the characteristics of Pluronic micellar assemblies can be easily varied [9, 10]. In consequence, it is possible to tune up the shape and size of the overall morphology of the hybrid nanomaterials [11, 12].

UV-visible studies are the best spectroscopic techniques employed to monitor the synthesis of Au-NPs on the basis of their surface plasmon resonance (SPR). The peak position, maximum absorbance, and band shape of the plasmon resonance band depend on the particle structure, size, geometry, polydispersity, and the surrounding medium [12, 13]. The surface plasmon property allows the use of Au-NPs for many applications in the bioscience and medical fields [1417].

Recent advances in colloidal chemistry and their applications in the biomedical field explore several new concepts of target drug delivery systems [18, 19]. Therefore, it is important to extend the characterization techniques due to the new applications of Au-NPs. For example, drug release from semisolid carriers is influenced by the rheological behaviour [20]. The linear and nonlinear rheological behaviors of semidiluted aqueous micellar solutions of P103 have been reported as function of temperature and concentration, where shear viscosity and storage modulus increased three orders of magnitude with increasing temperature. These changes are related to the spherical-to-polymer-like micellar transition where the length of the micelles and the entanglement number density augment with increasing temperature [21]. Materials that change their structure in response to environmental stimuli such as temperature () are called “intelligent” or “smart” materials. In this context, for many applications, it is important to understand the rheological properties of complex fluids containing Au-NPs, since rheology has a direct relationship with their microstructure [22].

In this work, the synthesis of Au-NPs using Pluronic P103 as reductant and soft template in order to design tuned hybrid gold/P103 nanomaterials of different sizes and shapes is reported. The effect of P103 concentration and synthesis temperature on kinetics, size, and morphology of gold nanoparticles were studied. In order to study the effect of synthesis temperatures (30°C to 50°C) on the formation of Au-NPs, a range was selected to induce significant dehydration of the P103 micelles. Additionally, rheological properties of hybrid gold/P103 nanomaterials aqueous solutions were analyzed to explore the effect of the size and shape of Au-NPs on their shear viscosity as function of P103 concentration and measured temperature.

2. Materials and Methods

2.1. Materials

Hydrogen tetrachloroaurate (III) hydrate (HAuCl4·3H2O; 99.9%) was purchased from Sigma-Aldrich. Triblock polymer P103 (PEO17PPO60PEO17) from BASF was used as received. Double distilled water was used for all preparations.

2.2. Synthesis of Au-NPs

Aqueous P103 solutions were prepared in vials by mixing the copolymer with water under agitation at room temperature for 12 h. Then, the vials were placed in a water bath at the synthesis temperatures (30, 35, 40, 43, 45, and 50°C) for 12 h to ensure stabilization of the P103 micelles. To avoid light degradation of samples, vials were covered with aluminum foil. Aqueous solutions (total mass of 20 g) of P103 (10, 15, and 20 wt.%) and HAuCl4 (2.0 mM) were placed in screw-capped glass bottles. The mixing ratio of HAuCl4 to P103 was 1/9, according to Sakai’s method [3]. Concentration of HAuCl4 was kept constant at 2.0 mM in all mixtures. Reaction mixtures were kept in a water thermostat bath (Julabo F12) during 5 h at the synthesis temperature under static conditions. Then, samples were cooled at room temperature and stored in darkness to avoid light degradation.

2.3. Methods

UV-Vis spectroscopy was used to measure the reduction of HAuCl4 and consequently the formation of gold nanoparticles by observing changes in the absorption spectra at 220 nm and 540 nm [2, 3]. Samples were measured with a GENESYS 6 UV-visible spectrometer (Thermo Spectronic).

The size and shape of gold nanoparticles were determined in a JEOL-JEM-2010 Transmission Electron Microscope (TEM) in the conventional transmission mode using an accelerating voltage of 80 kV. Samples were prepared by mounting a drop of solution on a carbon-coated Cu grid and allowing it to air-dry.

Steady simple shear tests were performed in an AR-G2 rotational stress-controlled rheometer (TA Instruments) with a cone-and-plate geometry of 2° and 60 mm in diameter. A humidification chamber was set around the geometry to minimize water evaporation from the samples. Temperature was controlled within ±0.1°C during measurements using a Peltier plate system. Measurements were carried out at 30, 34, 37, and 40°C. All rheological measurements were performed at least three times, reporting the average values. Simple shear rate sweeps were performed under steady state conditions from 1 to 100 s−1. Vials were placed in a water bath at the measured temperature for 12 h before running the test to ensure stabilization of the sample. For each test, about 2 mL of sample was carefully transferred to the rheometer plate, minimizing any possible destruction of the sample.

3. Results and Discussion

Figure 1 shows typical UV-visible scans of a sample prepared with a P103 aqueous solution at 10 wt.% and synthesis temperature of 30°C. Under these conditions P103 formed spherical micelles [9]. It can be noticed that aqueous solution of P103 did not show any absorption band in the visible region. However, as soon as HAuCl4 was added, three prominent peaks appeared at 220, 320, and 540 nm, due to the presence of ions, the ligand to metal charge transfer band (LMCT), and surface plasmon resonance (SPR) of Au-NPs, respectively [2, 3, 12, 23].

Their intensity variation is presented in Figure 2. The ions (220 nm peak) produced a maximum intensity with no sign of Au-NPs absorbance (540 nm). Then a sudden decrease in the intensity of the 220 nm peak with simultaneous increase in the 540 nm peak was observed due to the formation of Au-NPs. After 200 minutes, intensity of the three peaks remained constant, indicating the end of the reaction.

It has been proved that the P103 copolymer at low concentrations is very efficient forming nanoparticles from HAuCl4 in aqueous solutions in the absence of other reductant and stabilizer agents [2, 3]. However, synthesis of Au-NPs at the semidiluted aqueous solutions of P103, where a rich variety of micellar structures are formed as a function of temperature and concentration, has not been reported yet [9].

The effect of reaction temperature for various P103 concentrations on the synthesis of gold nanoparticles is shown in Figure 3. Maximum intensity absorbance () measured at 540 nm was plotted versus various reaction temperatures (30, 35, 40, 43, 45, and 50°C) at different P103 concentrations (10, 15, and 20 wt.%). At 30°C, increased with increasing P103 concentration due to the progressive growth of spherical micelles together with Au-NPs. These micelles increased the number of surface cavities (nucleation centers) that subsequently form Au-NPs.

Moreover, when the polymer concentration increased, it caused not only a large increment in absorbance, but also a slight shift on the absorption band to larger wavelength suggesting that nanoparticles size increased (see inset (a) in Figure 3). The excessive presence of polymer allows surface cavities to come together causing large nucleation centers; this behavior could be responsible for the increase in size of the Au-NPs [2, 23]. Therefore, higher concentrations not only facilitate the reduction due to the presence of more surface cavities but will simultaneously entrap all nucleating centers in their micellar phase. However, when independent Au-NPs separate from the micelles, they generate their own SPR [2, 3].

By increasing the synthesis temperature to 35°C, micelles exhibited changes related to the spherical-to-polymer-like micellar transition, where micelles’ length and the entanglement number density augmented [21]. Polymer-like micelles have a greater surface area than spherical micelles, which causes an increase of the active sites where the ions are mostly reduced. In consequence, an increment in Au-NPs can be observed as an increase in the UV-Vis spectra absorbance intensity [24]. On inset (b) in Figure 3, a band centered at ca. 548 nm was observed for 10 wt.% of P103, indicating the formation of spherical gold nanoparticles. When P103 concentration increased (15 wt.%), an additional broad band at ca. 740 nm appeared to have originated from the self-aggregation of independent large colloidal NPs produced by the growing nucleation center [2]. As it was discussed above, the polymer-like micelles increased their aggregation number, which causes these collisions and increase in their surface cavities, which allowed the formation of larger nanoparticles. Consequently, besides spherical particles, large anisotropic aggregates might exist in samples, as it will be further confirmed by TEM images.

It has been reported for P103 that CPT is reached at ca. 40°C, leading to a reversible phase separation of polymer rich and water rich phases, with increasing size of P103 micellar clusters (polymer aggregates) [8, 9]. In other words, at certain temperature, water becomes a poor solvent to the polymer, possibly due to the new and less-polar polymer conformation, causing the prevalence of the polymer-polymer interaction and the growth of polymer aggregates, which leads to phase separation [8]. Álvarez-Ramírez et al. [9] reported the temperature-composition phases diagram of P103/water, where P103 samples with concentrations of 10, 15, and 20 wt.% have a CPT of 39, 40, and 41°C, respectively.

In our study, regions below and above the CP show an increase in the intensity of absorbance. Micelles and polymer aggregates tend to merge to produce larger respective assemblies with greater surface area. That in turn enhances the reduction process and thereby increases the intensity. However, at CPT point, remained almost constant for three concentrations due to micelles starting to get dehydrated at around 40°C and achieved a predominantly hydrophobic environment [2]. The nondependence of with polymer concentration suggests that the nucleating centers remain constant. To study the effect of CPT on Au-NPs formation, absorbance was plotted against wavelength at 50°C (inset (c) in Figure 3). In this UV-Vis spectrum, it was possible to observe sharp peaks with maximum absorbance at ca. 540 nm. This behavior suggested that polymer aggregates facilitated the reduction reaction from Au (3) to Au (0). Other absorption bands were observed at ca. 725 nm. The presence of these bands was due to self-aggregation of independent large Au-NPs [25]. Interestingly, there is a dependence between temperature and the band associated to the self-aggregation of the NPs. Notice that at 50°C (above the CPT), the band shifts to lower wavelength values compared to the band at temperature at 35°C (below the CPT). This correlation is related to the shape of the micellar assemblies. Electron transmission microscope (TEM) observations helped us to understand the morphology of Au-NPs and micellar assemblies below and above the CPT.

TEM studies of the synthesized samples at 30°C demonstrated how the relationship between micelles and Au-NPs resulted in a soft template where small Au-NPs were arranged on the surface of micelles (hybrid gold/P103 nanomaterials). This effect is illustrated in Figure 4, where well defined spherical micelles carried Au-NPs of different length on their surfaces (hybrid gold nanomaterials). No independent Au-NPs were observed suggesting that Au-NPs grew on the surface cavities and used micelle surface as template to self-assemble. Au-NPs grew in size and became more prominent when a larger amount of P103 was used.

Figure 4(a) shows the results obtained where 10 wt.% of P103 was employed. Micelles formation loaded with tiny Au-NPs (TNPs) of 1-2 nm was observed (see Figure 4(g)). When P103 concentration augmented to 15 wt.%, micelles and nanoparticles increased in size, since spherical micelles with average diameters of 70 nm and TNPs of 4 nm were observed (Figures 4(b), 4(c), and 4(h)). For P103 at 20 wt.%, micelles grew again (ca. 120 nm) as well as TNPs reaching sizes up to 6 nm (Figures 4(d), 4(e), 4(f), and 4(i)). The magnified view of a single micellar aggregate showed that the surface was fully covered by TNPs (Figures 4(e) and 4(f)). TNPs synthesized in this way were often close to be monodisperse, because their growth was mainly controlled by the number of the produced nucleating centers [2, 26]. This feature makes them interesting for their possible role as catalysts [27] and for medical applications such as chemotherapy and diagnosis of cancer cells [28].

Most of the published work related to the self-assembled behavior of block copolymer micelles and Au-NPs is restricted to spherical or vesicular morphologies where mostly a uniform distribution of Au-NPs is observed, mainly attributed to electrostatic interactions on the micelle-solution interface. However, for many applications, it is equally important to introduce the Au-NPs in the core of the polymer-like micelles [29, 30] in order to obtain Au-NPs with different shapes. Under the same reaction conditions but at 35°C, no spherical micelles assemblies were observed (Figures 5(a) and 5(b)). Instead, polymer-like micelles were observed. For 10 wt.% of P103, TNPs covered the entire polymer-like micellar surface providing a dark contrast; however, some independent spherical Au-NPs with an average size of 9 nm were identified. These results can be attributed to the copolymer polydispersity, since it is known that they have a broad polydispersity distribution. When the same reaction was carried out at 15 wt.% of P103 (Figures 5(c) and 5(d)), large Au-NPs were observed (ca. 34 nm). For samples prepared with 20 wt.% of P103, some of Au-NPs presented shapes and sizes similar to P103 micelles (Figures 5(e) and 5(f)).

Above ca. 40°C, CPT is reached, therefore, for samples prepared at 50°C (over CPT) and 10 wt.% of P103 (Figures 6(a) and 6(b)); long strands (indicated with white arrows) were observed rather than polymer-like micelles; above CPT long strands were dehydrated and were mainly composed of polymer. The arrangement of these strands allowed the formation of semispherical Au-NPs with an average size of ca. 43 nm and anisotropic particles. However, an increase in the amount of P103 (15 wt.%) produced a great variety of morphologies; that is, spherical nanoparticles had a size of ca. 60 nm, while nanorods were ca. 270 nm (Figures 6(c) and 6(d)). By adding a larger amount of polymer the occurrence of polymer-polymer contacts was promoted allowing the polymer’s agglomeration (Figure 6(c), indicated by one white arrow), where these templates acted as nanoreactors producing different nanostructures. With the increase in P103 concentration (20 wt.%), polymer aggregates were larger allowing the formation of nanoparticles of the same build (Figures 6(e) and 6(f)).

Figure 7 shows zero-shear-rate viscosity as a function of rheological measurement temperature (RMT) of aqueous solution of P103 used as reference (20 wt.%) and solutions of hybrid gold/P103 nanomaterials synthesized at 30°C. Under these conditions, soft templates with TNPs on their surface were observed by TEM analysis (see Figure 4).

Hybrid solutions exhibited a Newtonian behavior under steady shear rate measurements from the range of 30°C to 40°C (see insets (a) and (b)), except to the gold/P103 solution at 20 wt.%, where a shear thinning behavior was detected at shear rates larger than 10 s−1 (inset (b)). Shear thinning behavior in colloidal solutions is due to an alignment with the stream lines flow, so the Au-NPs aggregates break up and the random polymer coils orientate to the direction of flow. Figure 7 shows that aqueous solution of P103 at 20 wt.% (reference solution) was more viscous than hybrid solutions. Hybrid nanomaterials viscosity decreased due to presence of nanoparticles which reduced the interaction between micelles. Rheology of hybrid gold/P103 nanomaterials depends greatly on the hydrodynamics forces that act on the surface of the aggregates. Fernández and coworkers depicted the zero-shear-rate viscosity as a function of temperature for 20 wt.% P103 solution; they reported that an increase in RMT increases the viscosity due to a micellar growth [21]. Here, viscosity of hybrid nanomaterials increased when the RMT augmented similarity to the reference solution. Notice that viscosity between 30°C and 34°C was practically constant for three hybrid materials. This behavior is also attributed to the presence of nanoparticles; at this temperatures they inhibited partially the interaction between spherical micelles, causing the viscosity to remain constant. On the other hand, when temperature increased from 34°C to 40°C, an increase in viscosity was observed due to the increase of the entanglement number density. The rheological properties of the P103 reference solution and hybrid nanomaterial solutions were highly dependent on temperature since the viscosity increased nearly two orders of magnitude. These changes are related to the spherical-to-polymer-like micellar transition, where the length of the micelles and the entanglement number density augment with increasing temperature [9]. Moreover, for the hybrid nanomaterials, it was clear that viscosity decreased when polymer concentration diminished; this fact allowed to infer that the viscosity was strongly influenced by the amount of the unreacted copolymer (residual P103).

As previously shown, the synthesis temperature modified shape and size of nanostructures. These structures affected viscosity of the hybrid gold/P103 nanomaterials. Figure 8 depicts variation of zero-shear-rate viscosity for hybrid solutions, prepared at 30°C, 35°C, and 50°C, versus RMT. The concentration of P103 remained constant at 15 wt.%. When RMT was 30°C, the viscosity was the same in all three cases. However, when the RMT increased to 34°C, the sample synthesized at 50°C showed a slight increase in viscosity, possibly by presence of polymer aggregates and nanoparticles of different shapes and big sizes (see inset in Figure 8). Abdelhalim and coworkers have reported a similar behavior, where their findings revealed that Au-NPs with larger particle sizes (50 nm) exhibited more viscosity than those with smaller particle sizes (10 and 20 nm) [20].

The same trend was observed at 37°C and 40°C. When temperature of P103 was increased, micelles grew progressively and they could interact with the gold nanoparticles forming clusters that reduced its flow, leading to an increase in viscosity.

On the other hand, the hybrid gold/P103 nanomaterials prepared at 30°C exhibited higher viscosity than those prepared at 35°C (Figure 8). This behavior was attributed to the fact that at 30°C the hydrophilic ends (superficial cavities) of polymeric templates are exposed to the surrounding medium; thus, increasing shear rate and temperature; these cavities interact with each other increasing the size of the micelles (see white arrow in inset of Figure 8). At the synthesis temperature of 35°C, well defined nanoparticles were formed, which was a consequence of the polymer-like micelles. If temperature increases, the micelles form is modified (which gradually dehydrates); thus the hydrophilic interactions between aggregates decrease and consequently the clusters fall apart, which causes a viscosity decrease.

4. Conclusions

The synthesis of gold nanoparticles (Au-NPs) using the Pluronic P103 as a reductant and soft template to design tuned hybrid gold/P103 nanomaterials was reported here. With the use of the triblock copolymer commercially known as Pluronic P103, it was possible to prepare tuned morphologies of gold hybrid nanomaterials. The amphiphilic character of the P103 allowed the formation of gold hybrid nanomaterials with different shapes and sizes. The results concluded that the final morphology of hybrid gold/P103 nanomaterials was entirely controlled by the form of micellar assemblies (i.e., spherical micelles, polymer-like micelles, and polymer aggregates), depending on whether they were below or above the cloud point temperature (CPT). Below the CPT, micelles underwent changes related to spherical-to-polymer-like micelles transition, where small Au-NPs arranged on the surface of micelles (which acts as soft templates) and independent Au-NPs were formed. On the contrary, above the CPT, polymer-like micelles were dehydrated; the arrangement of these structures allowed the formation of semispherical Au-NPs and anisotropic nanoparticles. An increase in the amount of P103 produced a great variety of morphologies, in the range of spherical, rhombic, ellipsoidal, and hexagonal.

Finally, rheological measurements of aqueous solutions of hybrid gold/P103 nanomaterials showed that viscosity was sensitive to the concentration of P103 and measured temperature. Viscosity decreased when polymer concentration diminished; however, increasing measured temperature resulted in an increase in viscosity by nearly two orders of magnitude. These changes were related to a spherical-to-polymer-like micellar transition. Moreover, the synthesis temperature modified the shape and size of the nanostructures. These structures affected the viscosity, where it was observed that viscosity increased due to the presence of nanoparticles of different shapes and sizes.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.


The authors are very grateful to Francisco Ruiz and Jaime Mendoza for technical support at TEM measurement at UNAM. This research was financially supported by Secretaria de Educación Pública (SEP), PROMEP/103.5/10/4104 México, and Benemérita Universidad Autónoma de Puebla, VIEP 2014.