Table of Contents Author Guidelines Submit a Manuscript
Journal of Nanomaterials
Volume 2016 (2016), Article ID 7597295, 7 pages
http://dx.doi.org/10.1155/2016/7597295
Research Article

Bactericide Effect of Silver Nanoparticles as a Final Irrigation Agent in Endodontics on Enterococcus faecalis: An Ex Vivo Study

1Doctorate Program in Engineering and Materials Science, Autonomous University of San Luis Potosí, Avenida Dr. Manuel Nava No. 2, Zona Universitaria, 78290 San Luis Potosí, SLP, Mexico
2Doctorate Program in Dental Science, Faculty of Dentistry, Autonomous University of San Luis Potosí, Avenida Dr. Manuel Nava No. 2, Zona Universitaria, 78290 San Luis Potosí, SLP, Mexico
3Faculty of Science, Autonomous University of San Luis Potosí, Avenida Dr. Salvador Nava S/N, Zona Universitaria, 78290 San Luis Potosí, SLP, Mexico
4Biology Cell and Ultrastructure Department, Biomedical Research Center, Faculty of Medicine, Autonomous University of Coahuila, 27000 Torreón, COAH, Mexico

Received 16 February 2016; Revised 13 April 2016; Accepted 10 May 2016

Academic Editor: Seunghan Oh

Copyright © 2016 Pedro-IV González-Luna et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The objective of this study was to determine the bactericidal effect of silver nanoparticles as a final irrigation agent in endodontics. This study included 120 uniradicular extracted dental organs inoculated with Enterococcus faecalis (E. faecalis) and organized into 4 groups: (A) 30 teeth irrigated with a dispersion of silver nanoparticles (537 μg/mL); (B) 30 teeth irrigated with a sodium hypochlorite solution (2.25%); (C) 30 teeth irrigated with a dispersion of silver nanoparticles (537 μg/mL) + EDTA (17%); and (D) 30 teeth with a saline solution. After the irrigation protocol, the samples were analyzed through a spectrophotometer to measure the bactericidal effect and scanning electron microscope and atomic force microscope in order to observe the presence of dental smear layer. The results showed that nanoparticles of 10 nm and the sodium hypochlorite at 2.25% were effective for eliminating E. faecalis, with no significant difference between them.