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Journal of Nanomaterials
Volume 2016, Article ID 7834657, 8 pages
Research Article

Label-Free Detection of Chondroitin Sulphate Proteoglycan 4 by a Polyaniline/Graphene Nanocomposite Functionalized Impedimetric Immunosensor

1Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China
2Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China
3Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA

Received 19 November 2015; Accepted 11 January 2016

Academic Editor: Hassan Karimi-Maleh

Copyright © 2016 JingJing Fu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The same cell culture supernatant was analyzed by a direct ELISA and a sandwich ELISA. For the direct ELISA, proteins in supernatant was immobilized into a 96-well microplate (Fisher Scientific, USA); while for sandwich ELISA, CSPG4-specific mAb was coated on the microplate.

  1. Supplementary Material