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Journal of Nanomaterials
Volume 2016, Article ID 8194516, 7 pages
Research Article

The Influence of Titanium Dioxide on Diamond-Like Carbon Biocompatibility for Dental Applications

1Laboratory of Biomedical Nanotechnology, University of Vale do Paraíba, São José dos Campos, SP, Brazil
2Associated Laboratory of Sensors and Materials, National Institute for Space Research, São José dos Campos, SP, Brazil
3Laboratory of Cell Biology and Tissue, University of Vale do Paraíba, São José dos Campos, SP, Brazil

Received 8 May 2016; Revised 15 August 2016; Accepted 23 August 2016

Academic Editor: P. Davide Cozzoli

Copyright © 2016 C. C. Wachesk et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The physical and chemical characteristics of diamond-like carbon (DLC) films make them suitable for implantable medical and odontological interests. Despite their good interactions with biological environment, incorporated nanoparticles can significantly enhance DLC properties. This manuscript studies the potential of titanium dioxide (TiO2) incorporated-DLC films in dental applications. In this scene, both osteoblasts attachment and spreading on the coatings and their corrosion characteristics in artificial saliva were investigated. The films were grown on 304 stainless steel substrates using plasma enhanced chemical vapor deposition. Raman scattering spectroscopy characterized the film structure. As the concentration of TiO2 increased, the films increased the osteoblast viability (MTT assay), becoming more thermodynamically favorable to cell spreading ( values became more negative). The increasing number of osteoblast nuclei indicates a higher adhesion between the cells and the films. The potentiodynamic polarization test in artificial saliva shows an increase in corrosion protection when TiO2 are present. These results show the potential use of TiO2-DLC films in implantable surfaces.