Antibacterial Activity of Partially Oxidized Ag/Au Nanoparticles against the Oral Pathogen Porphyromonas gingivalis W83

Figure 4

Growth curves of P. gingivalis W83 comparing the antibacterial activity of aerobically prepared nanoparticles with 0.5 mM AgNO₃. Bacterial growth over a 24-hour period was assessed by measuring the absorbance of cultures at 600 nm for specific intervals. P. gingivalis W83 ( ≈ 0.15) was challenged with water (400 μL), maltose coated Ag (≈0.2 nM, 400 μL; ) or glutathione capped Ag/Au (Au : Ag ≈ 0.2; ≈0.4 nM, 400 μL; ) nanoparticles, or 0.5 mM AgNO₃ (6.25 mM, 400 μL) in 5 mL total volume. Glutathione capping was done using method I. Error bars represent the standard deviation of three experiments.