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Journal of Nanomaterials
Volume 2017, Article ID 1287698, 12 pages
Research Article

New Class of Antimicrobial Agents: SBA-15 Silica Containing Anchored Copper Ions

1Department of Microelectronics and Nanotechnology, Czestochowa University of Technology, Al. Armii Krajowej 36, 42-201 Czestochowa, Poland
2H. Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, ul. Radzikowskiego 152, 31-342 Krakow, Poland
3Institute of Environmental Engineering, Czestochowa University of Technology, ul. Brzeznicka 60a, 42-200 Czestochowa, Poland
4Institute of Computer Science, Czestochowa University of Technology, ul. Dabrowskiego 69, 42-201 Czestochowa, Poland
5Institute of Mechanical Technologies, Czestochowa University of Technology, Al. Armii Krajowej 21, 42-201 Czestochowa, Poland
6Faculty of Computer Science and Materials Science, University of Silesia, Institute of Materials Science, Silesian Center for Education and Interdisciplinary Research, ul. 75 Pułku Piechoty 1A, 41-500 Chorzów, Poland

Correspondence should be addressed to Lukasz Laskowski; lp.zcp.kik@ikswoksal.zsakul

Received 9 September 2016; Revised 18 November 2016; Accepted 7 December 2016; Published 22 February 2017

Academic Editor: Mauro Pollini

Copyright © 2017 Lukasz Laskowski et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The paper is about a new class of antimicrobial functional nanomaterials. Proposed compounds are based on SBA-15 porous silica matrices and contain anchored copper ions. Thanks to the immobilization of functional groups the compounds are safer for environment than commonly used disinfectant agents. We prepared and examined silica based materials containing two concentrations of copper-containing groups: 10 and 5%. For the reference we prepared samples containing free-standing CuO molecules in the structure and checked their antimicrobial properties. Antibacterial effect of considered SBA-15-Cu material was tested on Escherichia coli bacteria. Antimicrobial tests were applied for the pure form of the material and as modifying agents for plastics. The obtained results showed that the sample with lower concentration of active copper-containing groups has stronger antimicrobial properties than the one with higher concentration of copper. Interestingly, silica containing free-standing CuO molecules has no antimicrobial properties. Considering the obtained results, we can conclude that the most probable antimicrobial mechanism in this case is an oxidation stress. When a plastic modifier is applied the material is enriched with bacterial inhibitory properties. It seems that SBA-15 silica containing low concentration of anchored copper ions is promising in terms of its antibacterial property and biomaterial potential for commercial use.

1. Introduction

It is known that the growth of many bacteria, fungi, and viruses can lead to the development of infectious disease. Thus, there is an ongoing research for the formation of low-cost, efficient, and easy to use antimicrobial surfaces and materials for many applications such as medicine and medical devices, linens and clothing, or coatings for home-used appliances (toilets, sinks, freezers, knives, etc.). Recently a substantial increase of using antimicrobial nanomaterials has been noticed. Metals like silver or copper can be toxic to bacteria, having antimicrobial activities at exceptionally low concentrations. Moreover, unlike other antimicrobial agents they are stable under conditions currently found in the industry allowing for their use as additives. Particularly, copper and silver based compounds have been widely used as antimicrobial agents [1] in many applications in agriculture, healthcare, and general industry. Nevertheless, using metallic copper or silver in industrial applications involves several challenges associated with the nature of the metal itself. Silver is relatively expensive, while copper is susceptible to corrosion process. Anyway, metal nanoparticles have become more and more popular. As it has been proven, such a form of silver or copper exhibits strong antimicrobial properties [24] which becomes stronger with the decrease in their hydrodynamic size [5]. Therefore many research teams are struggling to get the smallest metal nanoparticles [6]. In the case of copper, nanoparticles can be dissolved faster in comparison with larger particles, releasing higher amount of Cu ions [7]. Copper biocide behaviour is triggered by the metal reduction potential. Copper plays a role of catalyst and causes one-electron reduction of oxygen atoms [1]. In the presence of either superoxide or other reducing agents such as ascorbic acid, Cu can be reduced to Cu catalyzing the formation of hydroxyl radicals from hydrogen peroxide [8, 9]. This can induce an oxidative stress damaging cellular proteins, lipids, and DNA [1012] or even trigger proinflammatory signaling cascades into the cell that are able to induce its programmed death [13]. Nevertheless, recent studies indicate antibacterial property through a mechanism of damaging bacterial cell membranes by the generation of reactive oxygen species but surprisingly preserving the integrity of bacterial genomic DNA [14].

Moreover some toxic mechanisms can depend on cellular characteristics at the nanoscale and the particle size itself can be crucial in this case [8]. A good example of this mechanism can be the one called “the Trojan horse”: direct incorporation of nanoparticles into the cell via endocytotic mechanisms causes subsequently ions release within the cells by nanoparticle dissolution. This results in massive oxidative stress and cell’s death [1517].

It seems that nanoparticles have the potential to extend the range of antimicrobial application of copper. Recently their addition to a polymer matrix to obtain a composite material is very promising. Important advantage of such materials is the extremely low amount of filler needed to achieve desired features. Required amounts can be one or even two orders of magnitude lower than conventional microfillers [18, 19]. For example, copper was impregnated on the surface of cotton fibers, latex, and other polymeric materials [20]. Interesting approach is copper incorporation by plasma immersion ion implantation to produce antibacterial polyethylene surface, as reported in [21]. In [22] authors show the process of ionic crosslinking of alginate chains within the cotton cellulose fibers with Cu ions during production of copper alginate-cotton cellulose (CACC) composite fibers.

Interesting study was presented in [23], where chitosan nanoparticle was loaded with copper ions. Authors observed significant enhancement of their features compared to those of chitosan nanoparticles and copper ions used separately.

The antimicrobial mechanism of supported copper nanoparticles is reported similarly to the mechanism of the metal ions [19, 24]: the metal nanoparticle release and accumulation in the bacteria’s cell or the metal ions releasing from the particles and their action [11]. Nevertheless majority of authors indicated this second mechanism as leading in antibacterial action of metals on supports [1, 2528]. In [29, 30] authors confirmed that antimicrobial effect of these composed materials is related to the metal ion releases rather than to the leaching of the whole metal nanoparticles.

The antimicrobial activities of metal nanostructures are mostly restricted to strains of pathogenic microorganisms and rarely extended to other microorganisms [31, 32]. Nevertheless, the impact of nanoparticles on environmental accumulation, especially on microbial communities residing in land fields or bodies of water, which are critical to environmental recycling, is unknown. Therefore, it is vital to limit the migration of antimicrobial agents to the environment. It would be advantageous to design an antimicrobial material that is active only locally with low migration of the agent from the working place.

We propose different approach to the problem using copper-containing antimicrobial units homogeneously distributed inside SBA-15 mesoporous silica matrix [33, 34] and anchored inside pores.

The specimen proposed here shows strong antimicrobial action against E. coli bacteria. The biocidal mechanism revealed by copper-containing silica introduced by the authors seems to be different than that proposed elsewhere for copper species known from literature. Moreover they operate locally with significantly limited antimicrobial agent depletion (metal ions anchored to silica surface), so possibility of metal migration outside the active area is highly reduced.

We prepared SBA-15 mesoporous silica containing copper ions bounded inside pores via propylphosphonate units and also SBA-15 containing free-standing copper oxide molecules inside pores as a reference to test this hypothesis. We utilized transmission electron microscopy (TEM), EDX quantitative elemental analysis, and Raman spectroscopy supported by numerical simulations to find characterization of the materials molecular structure. It is worth noting that we show molecular structure’s characterization only for SBA-15 containing free-standing copper oxide molecules while silica containing copper ions bounded inside pores via propylphosphonate units were thoroughly analyzed in [35].

Furthermore, we examined the antibacterial properties of considered materials using well established microbiological assay. Discovered antimicrobial properties of the samples enabled us to raise a hypothesis about the most probable bacteria elimination mechanism.

To confirm antimicrobial action mechanism we additionally carried out elemental analysis to show hydrogen content in the species and atomic absorption spectroscopy to evaluate copper ion migration to the medium.

Moreover we examined bacterial inhibitory properties of two plastics modified by functional nanomaterial: high density polyethylene (HDPE) and polyurethane (PU).

2. Materials and Methods

Four batches of samples were investigated with regard of their antimicrobial properties:(i)Mesoporous silica SBA-15 containing copper ions bounded inside pores via propylphosphonate units (SBA-prop-POOCu) in concentrations 10% and 5%—main investigated material(ii)Mesoporous silica SBA-15 containing nonbounded copper oxide molecules inside pores (SBA-CuO) in concentrations 10% and 5%(iii)High density polyethylene modified with SBA-prop-POOCu (HDPE-Cu) containing 0.5 and 1% of modifier(iv)Polyurethane modified with SBA-prop-POOCu (PU-Cu) containing 0.5 and 1% of modifier

The structure of the samples containing anchored copper ions (SBA-prop-POOCu) and free-standing copper oxide molecules (SBA-CuO) can be seen in Figure 1.

Figure 1: Schematic representation of the SBA-15 silica containing copper ions anchored via propylphosphonate unit with magnification of the copper-containing unit anchored to the pore (a) and SBA-15 silica containing free-standing copper oxide molecules (b).

For bactericidal tests we used SBA-15 mesoporous silica containing propylphosphonate units (SBA-prop-PO(OH)) as a reference sample for copper-containing bioactive materials while references for modified plastics were pure HDPE and PU.

In the case of Raman spectroscopy of SBA-CuO, pure SBA-15 silica was a reference to probe a molecular structure.

2.1. Methodology

The main goal of the present work was to examine antibacterial properties of SBA-prop-POOCu and determine the mechanism of bacteria elimination.

Proposed methodology is based on comparison of antimicrobial properties of the sample containing immobilized copper-containing groups (SBA-prop-PO(OH)) with the sample that contains nonbounded copper oxide molecules (SBA-CuO). We compared samples containing exactly the same molar concentration of copper-containing groups (anchored copper phosphonate or free copper oxide). In both cases the copper ions are coordinated by oxygen atoms. As far as biocidal properties are concerned, the main difference between samples lays in copper-containing units mobility. In the case of SBA-prop-POOCu migration of copper-containing units is strongly limited due to bounding to silica walls. Thus the probability of bacteria cells penetration is also limited. Opposite situation can be found in the case of SBA-CuO; copper oxide molecules can migrate and penetrate the bacteria cells.

To confirm our statement about limited migration of copper from SBA-prop-POOCu samples, we carried out atomic absorption spectroscopy. The experiment was performed in the following way: the materials that underwent antimicrobial tests (SBA-prop-POOCu 5%, SBA-prop-POOCu 10%, SBA-CuO 5%, and SBA-CuO 10%) were added to the same nutrient broth that was used for antimicrobial tests in amount of 1 g/L (the highest specimens’ concentration used for antimicrobial tests; see next text) and rigorously mixed in magnetic stirrer in the temperature of 37°C (the same temperature as for antimicrobial tests). After the time of 1 h, 28 h, 48 h, and 72 h we poured out some amount of suspension and filtered it out using filtering funnel with gradation of 4. The obtained clear solution was investigated under AAS with regard to copper content.

Another difference between the samples lies in the surroundings of copper ions. For SBA-prop-POOCu copper surrounded by six copper ions creates octahedral environment with further oxygen atoms in crystalline lattice (see next chapter). In this case silica pores play the role of oxygen tanks. On the other hand for SBA-CuO specimen copper is coordinated with only two oxygen atoms with no presence of oxygen crystalline lattice. In this case silica pores do not contain significant quantity of oxygen. When pathogens elimination caused by the bacteria cells penetration is considered, we should observe much stronger biocidal properties of SBA-CuO, while in the case of bacteria elimination caused by oxidation stress SBA-prop-POOCu should be much more efficient. To confirm in explicit way the differences in oxygen content for all samples investigated we carried out additional elemental analysis. The results were juxtaposed with theoretical percentage content of oxygen (calculated on the base of atomic composition) and results for reference samples were mesoporous silica containing propylphosphonate units not activated by copper (SBA-prop-PO(OH)). During theoretical content calculation we omitted hydrogen atoms in the surface units; it is impossible to estimate their content in the structure. For the reason of low atomic mass of hydroxyl atoms the error caused by this simplification can be neglected.

2.2. Samples Preparation

Mesoporous silica SBA-15 containing copper ions bounded inside pores via propylphosphonate units (SBA-prop-POOCu) and reference material (SBA-prop-PO(OH)) were prepared according to procedure described in [35]. Obtained materials contained two molar concentrations of the copper-containing unit: 10% and 5%. For 10% concentration of the phosphonate units proportions between tetraethyl orthosilicate (TEOS) and PhosphonatePropylTriEthoxySilane (PPTS) were 1 : 9, while for 5% they were 1 : 19.

Samples containing free-standing copper oxide molecules inside pores were prepared by calcination of the SBA-prop-PO(OH) in air in 1100°C.

Polyethylene sample (Hostalen GC 7260, manufactured by Lyondell and Basell) modified by SBA-prop-POOCu was prepared by mechanical stirring of granulated polymer with modifier and then extruded with the use of continuous extruder. Bioactive agent was added in amount of 0.5 and 1% of mass. Samples were obtained on the extruder KRAUSS MAFFEI KM65-160C1. Applied extrusion parameters for all samples were as follows: maximal pressure in the plasticiser part, 60 MPa; extrusion time, 0.6 s; clamps pressure, 30 MPa; clamps time, 28 s; cooling time, 15 s; dozing time, 6.6 s; form closing force, 650 kN; form temperature, 40°C; extrusion temperature, 195°C; screw rotational speed, 250 mm/s. It must be stressed that decomposition temperature of organic moiety in the sample (propyl chain) is 320°C, while phosphonic acid groups decompose at 320°C. Thus there is no possibility of modifier decomposition during the process. After this process the material was casted into discs. Granulate of composites was obtained using the rotor mill. Average diameter of the grains was 2 mm.

In order to prepare polyurethane modified by 0.5 and 1% (in weight proportions) of SBA-prop-POOCu we used commercially available two-compound PU resin (MoldStar 321 manufactured by Smooth-on ltd.). In the first step we mixed polyol with appropriate weight of modifier. Next we added isocyanate in exactly the same amount (in weight proportion) as polyol. This was mixed mechanically for about one minute. The mixture was deaerated in the vacuum chamber for 2 minutes under the pressure of 150 μmHg. Then we casted material in the form of ring with diameter of 10 cm and thickness of 1 cm using silicone form. After polycondensation (4 hours) the material was grinded into the grains with diameter about 2 mm.

2.3. Characterization Methods

TEM imaging was carried out using the FEI Tecnai G2 20 X-TWIN electron microscope, equipped with emission source LaB6, CCD camera FEI Eagle 2K and X-ray microanalyzer EDX.

Raman spectroscopy measurements were carried out at room temperature in the wavelength range from 300 to 4000 cm−1. The Raman spectrometer (Nicolet Almega XR) was equipped with a Nd:YAG laser. The experiments were carried out at 532 nm and the laser was operated at a power level of 40 mW. The spectral resolution of the spectrometer was 1 cm−1. As SBA-15 silica is a noncrystalline solid, Raman scattering resolution is rather poor. To increase spectrum quality powder was compressed to pellets, so each spectrum was a result of 10 scans.

In order to determinate the oxygen quantity inside samples elemental analysis was carried out with the use of vario EL cube elemental analyzer upgraded to oxygen determination with a pyrolysis in 1150°C.

The copper content determination was evaluated by atomic absorption spectroscopy (AAS) with use of Shimadzu model AA-680 atomic absorption spectrometer having hollow cathode lamp and a deuterium background with an air-acetylene flame. The lamp current was set to 6 mA. Measurements were carried out in the integrated absorbance mode at 324.8 nm, using slit width of 0.7.

2.4. Numerical Simulations

For considered samples we performed quantum chemistry simulations to support Raman spectroscopy and identify vibrational modes observed in the Raman spectra. Applied procedure was described in [34]. Considering the fact that SBA-prop-POOCu sample’s structural investigation was presented in other papers [34, 35] we limited our presentation to numerical model for SBA-CuO samples only.

We used the bicyclic 5-6-s cluster model [36] that would be applied as a representative cluster model of SBA-15 silica. This system was used as a starting point for our simulations. Copper(II) oxide molecule was placed in the distance of Van der Waals interaction. The multiplicity of SBA-15 model molecule and copper oxide molecule were different. The geometry of the model was fully optimized at the level of B3LYP [37, 38] with the 6-311G(d,p) basis set. After geometry optimization, Raman vibrational modes were calculated. For calculation of harmonic vibrational frequencies the same method and basis set were used.

All of these theoretical calculations were carried out using the GAUSSIAN 09 package [39] with default convergence criteria applied. The assignment of the calculated Raman bands was done on the basis of PED analysis [40, 41] and aided by the animation option of the GaussView 5.0 graphical interface for Gaussian programs [42], which gave a visual representation of the vibrational modes shape. Then VEDA software [43] was applied to proceed with the PED analysis. By combining the results of the visualization with potential energy distribution (PED) we obtained very accurate description of the molecules vibrations.

2.5. Antibacterial Tests

The antimicrobial activity of the copper-containing silica nanomaterials was tested against the Gram-negative bacteria Escherichia coli (ATCC 8099, Rockville MD, USA). To carry out macrodilution test [44] for SBA-prop-POOCu and SBA-CuO powders we prepared ten sets of 10 mL nutrient broth medium containing nanomaterial with concentration from 100 to 1000 μg/mL. Each set was inoculated aseptically with 108 CFU/mL of the bacterial suspension. The inoculated sets were incubated at 37°C for 24 h. Each experiment was carried out in triplicate to ensure reproducibility. Turbidity of the media was treated as a measure of bacterial growth. For better evaluation of the bacterial concentration differentiating medium was used (ChromoCult, Merck). The minimum bactericidal concentration (MBC) [45] was evaluated by measuring the turbidity of bacterial strains exposed to different nanoparticles’ concentrations. Control experiments were also run parallel to investigate the antibacterial activities of nutrient broth medium with pure silica matrix.

Antibacterial tests for the plastics modified by SBA-prop-POOCu were prepared in different way. In this case we decided to maintain constant conditions (the same amount of nutrient broth medium and modified plastic in the form of pellets and the same concentration of bacterial suspension) for investigated materials: HDPE and PU (both polymers modified with 0.5 and 1% of bactericidal agent and in its pure form as a reference). So we placed 4 grams of samples pellets in the Petri dishes and added 10 mL nutrient broth medium inoculated aseptically with  CFU/mL of the bacterial suspension. The inoculated sets were incubated at 37°C for 48 h. To estimate inhibitory properties of the sample we counted bacteria colonies in the Petri dishes with samples and compare with results obtained for nonmodified polymers. The mean reported for each polymeric sample was based on ten replicates.

3. Result and Discussion

3.1. Structural Investigation and Molecular Structure Probing

The structure of SBA-prop-POOCu material had been thoroughly investigated in our previous work [35], so we showed investigation results only for SBA-CuO sample.

Main features of the structure were provided by the transmission electron microscopy (TEM). TEM images of the SBA-CuO, observed along the direction of the pore array and perpendicularly to the pore array, can be seen in Figure 2.

Figure 2: TEM images of the SBA-15 silica containing free-standing copper oxide molecules (SBA-CuO) observed along the direction of the pore array (a) and perpendicularly to the pore array (b). The grains sizes can be easily estimated as varying mainly from 50 to 300 nm.

As can be easily noticed, TEM microscopy confirms that investigated sample has well ordered, hexagonally arranged pore array pattern nanostructure. Also pore diameter can be roughly measured as about 5 nm. Moreover, any impurities or copper-containing groups agglomerations cannot be seen.

The quantitative EDX analysis was carried out for copper oxide containing silica samples (containing 5 and 10% of CuO) in order to confirm the chemical composition of the samples. The EDX spectrum is shown in Figure 3 and quantification results are presented in the Table 1.

Table 1: EDX quantification results of SBA-15 mesoporous silica powder containing 5 and 10% of copper oxide units (SBA-CuO).
Figure 3: EDX spectrum of SBA-15 silica containing 5% (a) and 10% (b) free-standing copper oxide molecules. For better visibility region between 2 and 8 keV (no peaks presence) has been cut.

The presence of nickel (Ni) in the EDX spectra is attributable to the preparation technique, in which samples were placed on a nickel grid. The presence of carbon (C) is connected with vacuum chamber contamination (using a carbon tape for placing the grids). Oxygen (O) amount is not reliable, because of air remains in the chamber.

In this case we used EDX measurement for finding proportion between key elements in our samples: silicon and copper. Even in the case of contamination, which is common for this technique, the proportions between the elements listed before remain unchanged and results are reliable.

It is clearly seen that the SBA-CuO samples have almost assumed quantity of copper; the molar ratio of silicon to copper in the case of samples containing 5 and 10% of CuO is equal to 21.69 and 12.20, respectively. Minor deficiency of copper is probably caused by incorporation of propylphosphonic acid in silica walls in initial material before calcination and also by migration of free-standing copper oxide units.

We did not notice presence of phosphorus, which confirmed the correctness of the calcination process.

To identify bounds inside SBA-CuO samples and unambiguously confirm assumed molecular structure we carried out Raman spectroscopy supported by numerical simulations. Obtained theoretical model allowed vibrations to be identified in explicit way. Juxtaposition of the Raman spectra for model molecule, SBA-15 mesoporous silica (reference material), and silica containing copper oxide molecules can be seen in Figure 4. We present here low frequency spectra (from 200 to 1200 cm−1), only for the range in which we can observe significant Raman features.

Figure 4: The juxtaposition of the Raman spectra for silica containing copper oxide units; theoretic and experimental data and pure SBA-15 mesoporous silica. The vibrational mode characteristic for copper oxide is marked as colour bands.

We obtained good correlation between theoretical and experimental data, which confirms correctness of DFT simulations. The silica support exhibits Raman features at 370 and 560 cm−1 (deformation of siloxane rings), 770 cm−1 (stretching of siloxane bridges), and 1000 cm−1 (bending of surface Si-H bounds). Absence of typical vibrational models of hydroxy units (1200 cm−1) [46, 47] is caused by silanation of silica matrix during synthesis process. Applied theoretical model also had no silanol units.

Considering all abovementioned analysis we can be sure that the molecular structures of the investigated powders are in accordance with our expectations.

3.2. Antimicrobial Tests
3.2.1. Antimicrobial Activity of Pure Functional Powders

Antimicrobial activity of copper ions bounded within the pores of SBA-15 silica matrix (SBA-prop-POOCu) was analyzed against bacterial strain of Escherichia coli. We tested nanomaterials with two molar concentrations of copper-containing units: 10 and 5% (samples named SBA-prop-POOCu10 and SBA-prop-POOCu5, respectively).

Obtained results were surprising for us. While specimen containing 10% of copper-containing units showed quite strong antibacterial activity, specimen containing 5% of anchored copper ions revealed significantly stronger antimicrobial properties. The results of macrodilution tests was presented in Figure 5. For these tests reference material was SBA-15 silica containing pure anchoring groups, propylphosphonate (control sample).

Figure 5: Macrodilution antimicrobial tests against Escherichia coli bacteria for SBA-15 silica containing anchored copper ions in molar concentration of 10% (a) and 5% (b). Control sample is SBA-15 silica containing pure propylphosphonate units (precursor of SBA-prop-POOCu).

For the SBA-prop-POOCu10 sample MBC was found as 700 μg/mL. This result was comparable to commercially used nanosilver [48] and not much worse than silica-silver core-shell systems [49]. It is worth noting that investigated species included only 10% of functional copper-containing groups (in molar concentration). Taking into consideration the elemental composition of the specimen, MBC of copper ions was 57 μg/mL. This result is better than that obtained for pure copper oxide nanoparticles [50]. Additionally, our material has important property; functional copper-containing units are immobilized in the matrix and their migration and depleting is strongly limited.

SBA-prop-POOCu5 sample has much stronger bactericidal properties than SBA-prop-POOCu10 although it contains 2 times lower molar concentration of functional groups. For this sample we obtained MBC 300 μg/mL. Taking into consideration molar concentration of functional groups equal to 5% we obtained MBC equal to 12,4 μg/mL which is comparable with the best available biocidal agents.

The fact of bactericidal activity increasing with decreasing of functional groups concentration is a key to understanding the bacteria elimination mechanism. However the bactericidal action mechanism of metal nanoparticles is still not well understood. In literature we can find various modes of nanoparticles antimicrobial action [4, 5155]. Interestingly, each mechanism proposed in literature assumes diffusion of the active particle into the cell or direct contact of metal with cell membrane [56]. The mechanism of antimicrobial action of SBA-prop-POOCu active material seems to be different. First of all, copper-containing units are placed inside silica pores. Pores diameter is only 4.92 nm [35] which is much lower than the size of Escherichia coli bacteria. Moreover, samples used for antimicrobial tests have a form of fine powder in each case, with grains varying mainly from 50 to 300 nm, (see: 2) which is much smaller than bacteria’s cell. Thus the direct contact between bacteria cell and copper-containing groups is rather unlikely.

To confirm presented hypothesis, we carried out antibacterial tests for the samples containing nonanchored copper oxide, SBA-CuO (also in this case we investigated samples containing two molar concentrations of CuO molecules: 10 and 5%). Antimicrobial tests sets as for the case of SBA-prop-POOCu revealed that both SBA-CuO samples are ambient for Escherichia coli bacteria. Even the concentration of 1500 μg/mL (both SBA-CuO10 and SBA-CuO5) does not show any antibacterial properties.

It is highly probable that copper ions migration into nutrient broth medium is strongly limited in the case of SBA-prop-POOCu, while SBA-CuO can migrate into the medium. Obtained AAS results revealed the level of ions migration into the medium. AAS results were shown in the plot (Figure 6) and additionally juxtaposed in the Table 2.

Table 2: Juxtaposition of the content of trace copper in the nutrient broth medium after assumed steering time for the samples investigated under antimicrobial tests. Initial suspension contains concentration of the specimen in the broth medium of 1 g/L.
Figure 6: The content of trace copper in the nutrient broth medium as a function of steering time for the samples investigated under antimicrobial tests. Initial suspension contains concentration of the specimen in the broth medium of 1 g/L.

As can be clearly seen, migration of copper from every species investigated to the medium is rather low. Even in the case of the specimen containing nonbounded copper oxide molecules, after 24 h copper concentration in the nutrient broth medium seems to be too low to reach MIC against E. coli bacteria [57]. Nevertheless, for SBA-15 silica containing bounded copper ions (SBA-prop-POOCu) it is significantly lower than that for SBA-CuO. Looking at these results we can say that bacteria elimination mechanism of copper-containing silica is not connected with copper ions release into the broth medium.

This leads to the conclusion that antimicrobial action of such compounds (materials containing homogeneously distributed copper-containing units) is not connected with direct interaction of copper-containing molecules with a bacteria cell.

Therefore we can conclude that antimicrobial action of SBA-prop-POOCu seems to be involved in environment of copper ions, not copper ions themselves. This hypothesis explains well inverted dependence between molar concentration of functional groups and bactericidal activity of the species. As we showed on the base of magnetic research (not yet published), copper ions inside SBA-POOCu10 create distorted tetrahedral environment; each Cu is surrounded by four oxygen atoms. Unlike SBA-POOCu10, in the case of silica containing 5% of bounded copper-containing units, each copper ion is coordinated by six oxygen atoms, creating distorted octahedral environment. Distortion is caused by propylphosphonate anchoring groups. Such a difference in copper ion’s configurations is connected with distance between functional units in species containing different molar concentration of copper-containing groups. In the case of SBA-POOCu10 copper phosphonate groups are relatively close to each other and the most energetically favourable is tetrahedral configuration, whereas the SBA-POOCu5 copper-containing units are placed sparsely which involves octahedral configuration of copper ions. In both cases coordinating oxygen atoms creates local crystalline structure, which can be seen even in SQUID magnetometry. As a consequence, different amount of oxygen is accommodated inside the SBA-15 support pores. Much higher concentration of oxygen can be found in SBA-POOCu5 sample. Also AAS revealed differences in percentage content of oxygen in our sample. The results were juxtaposed with theoretical percentage content of oxygen (taken from structural assumption) and oxygen content in reference samples: mesoporous silica containing propylphosphonate units not activated by copper (SBA-POOH). Obtained results were juxtaposed in the Table 3.

Table 3: Elemental analysis results indicating percentage content of oxygen in the samples investigated.

As can be clearly seen for both reference samples and silica containing copper oxide molecules amount of oxygen seems to be close to theoretically calculated value. On the other hand amount of oxygen for the samples with copper ions bounded via propylphosphonate units is significantly higher than assumed. Moreover, oxygen amount inside sample containing 5% of bounded copper ions is higher than that for the sample containing 10% of copper phosphonate units. Assuming the same structure of the matrix (SBA-15 type silica) we can explain these differences as caused by oxygen trapped inside pores. It seems that this confirms in an explicit way our thesis about the presence of oxygen crystalline lattice inside pores.

Concerning all the above, it seems to be confirmed that bactericidal activity of SBA-prop-POOCu is connected with oxygen accumulated inside silica pores.

The antimicrobial mechanism proposed here is a result of direct contact between activated SBA-15 silica grains and bacteria’s cell. This causes direct interaction with oxygen stored inside silica pores with bacteria’s cell membrane. Copper centres inside silica pores can play a role of catalyst and cause one-electron reduction of oxygen atoms. Presence of superoxide Cu that can be reduced to Cu catalyzes the formation of hydroxyl radicals from hydrogen peroxide [9]. The hydroxyl radical is the most powerful oxidizing radical reacting with practically every biological molecule [58]. It can cause oxidative damage by abstracting the hydrogen both from an amino-bearing carbon to form a carbon centred protein radical and from an unsaturated fatty acid to form a lipid radical [56]. Thus bacteria’s cell membrane has a contact with highly reactive oxygen, which can cause its damage and finally death. Described mechanism was depicted in Figure 7.

Figure 7: Schematic presentation of antimicrobial action of SBA-prop-POOCu specimen against E. coli bacteria.

The antimicrobial action attributed to SBA-prop-POOCu involves a few advantages of these species. Migration and depleting of antimicrobial units are strongly limited, so the proposed biomaterials can operate locally and can be used as disinfectant with long-time local action, unlike antibiotics.

3.2.2. Antimicrobial Activity of Modified Polymers

Antibacterial activity of polymers modified by SBA-prop-POOCu5 was shown in Figure 8. After 48 hours of incubation we counted colony forming units in Petri dish. We tested high density polyurethane modified by copper-containing agent in amount of 0.5 and 1% of weight (HDPE-Cu 0.5 and HDPE-Cu 1) and polyurethane modified in the same concentration of biocidal specimen (PU-Cu 0.5 and PU-Cu 1). To estimate inhibitory properties of the plastics we juxtaposed obtained results with activity of pure polymers (high density polyethylene, HDPE, and polyurethane, PU).

Figure 8: Counts of bacteria colony forming units (CFU) (Escherichia coli) for polymers modified by SBA-POOCu5 in different mass concentration. High density polyethylene (a) and polyurethane (b).

As can be clearly seen, modified polymers inhibit growth of the Escherichia coli bacteria. In the case of HDPE we obtained  CFU after 48 hours of incubation. Looking at results for modified HDPE we see significant decreasing of the CFU numbers after the same time of incubation. In the case of HDPE-Cu 0.5 it is  CFU (bacterial growth fallen by 44%), while for HDPE-Cu 1 we counted  CFU (bacterial growth had fallen by 77%).

Similar results were obtained for modified polyurethane. Reference sample (PU) showed  CFU after 48 hours of incubation, while modified samples revealed inhibitory properties; we counted of CF for PU-Cu 0.5 (bacterial multiplication fallen by 49%) and of CF for PU-Cu 1 (bacterial multiplication fallen by 84%).

Antimicrobial action of polymers filled by SBA-POOCu is connected with silica particles placed on the polymers surface. This fact seems to explain why antimicrobial action in this case is weaker; only limited amount of modifier added to polymer can be placed in the surface after polycondensation process.

Nevertheless it is worth noticing that modification of the commercially used polymers with a small amount of proposed antimicrobial nanomaterial containing low concentration of anchored copper-containing units can enrich them with bacterial inhibitory properties. Moreover such modified polymers are thermally stable and modification can be carried out in easy way during polycondensation or melting process [59].

4. Conclusion

We presented a new class of antimicrobial nanomaterials: SBA-15 mesoporous silica containing copper ions bounded inside pores via propylphosphonate units. Proposed materials showed strong antibacterial action against Escherichia coli bacteria. Moreover, antimicrobial properties increase with decreasing of copper-containing concentration in the SBA-15 support. Taking into consideration the content of copper ions, specimen with the molar concentration of 5% of copper phosphonate units exhibited better antibacterial action than the best commercially available disinfectant materials based on copper or silver.

To reveal the mechanism of its antibacterial action we carried out comparative biological investigations with SBA-15 silica containing free-standing copper oxide molecules. Before biological research we had thoroughly characterized the molecular structure of the species to be sure that their composition is exactly the same as we assumed.

On the base of obtained results and molecular configuration of copper-containing groups we concluded that the mechanism of antimicrobial action is oxidative stress.

We showed that modification of the polymers with copper-containing antimicrobial material enriches them with inhibitory properties towards Escherichia coli bacteria.

All of this seems to be really important as far as potential applications of the SBA-prop-POOCu are concerned.

Their strong antimicrobial character can be used as the material that destroys pathogens (antimicrobial) or stabilizes bacteria multiplication (biostatic). Anchoring of the functional groups causes the antimicrobial agent migration to be strongly limited. This makes the species safe for ecofriendly microorganisms. Possibility of using them as polymeric materials modifiers opens the doors to application of these materials to dental fillings or manufacturing antimicrobial switchers, parts of beds, or even toilet seats. Also fabrics or medical dressings can be modified by the proposed material. Wide application potential makes our materials extremely interesting both from commercial and from scientific point of view.

Competing Interests

The authors declare that they have no competing interests.


Financial support for this investigation has been provided by the National Centre of Sciences (Grant no. 2011/03/D/ST5/05996).


  1. J. A. Lemire, J. J. Harrison, and R. J. Turner, “Antimicrobial activity of metals: mechanisms, molecular targets and applications,” Nature Reviews Microbiology, vol. 11, no. 6, pp. 371–384, 2013. View at Publisher · View at Google Scholar · View at Scopus
  2. J. Pulit-Prociak and M. Banach, “Silver nanoparticles—a material of the future…?” Open Chemistry, vol. 14, no. 1, pp. 76–91, 2016. View at Publisher · View at Google Scholar · View at Scopus
  3. C. A. Dos Santos, M. M. Seckler, A. P. Ingle et al., “Silver nanoparticles: therapeutical uses, toxicity, and safety issues,” Journal of Pharmaceutical Sciences, vol. 103, no. 7, pp. 1931–1944, 2014. View at Publisher · View at Google Scholar
  4. C. Khurana, P. Sharma, O. P. Pandey, and B. Chudasama, “Synergistic effect of metal nanoparticles on the antimicrobial activities of antibiotics against biorecycling Microbes,” Journal of Materials Science and Technology, vol. 32, no. 6, pp. 524–532, 2015. View at Publisher · View at Google Scholar · View at Scopus
  5. C. Khurana, A. K. Vala, N. Andhariya, O. P. Pandey, and B. Chudasama, “Antibacterial activity of silver: the role of hydrodynamic particle size at nanoscale,” Journal of Biomedical Materials Research Part A, vol. 102, no. 10, pp. 3361–3368, 2014. View at Publisher · View at Google Scholar · View at Scopus
  6. A. Azam, A. S. Ahmed, M. Oves, M. S. Khan, and A. Memic, “Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and-negative bacterial strains,” International Journal of Nanomedicine, vol. 7, pp. 3527–3535, 2012. View at Publisher · View at Google Scholar · View at Scopus
  7. H. L. Karlsson, P. Cronholm, Y. Hedberg et al., “Cell membrane damage and protein interaction induced by copper containing nanoparticles—importance of the metal release process,” Toxicology, vol. 313, no. 1, pp. 59–69, 2013. View at Publisher · View at Google Scholar · View at Scopus
  8. L. M. Gaetke and C. K. Chow, “Copper toxicity, oxidative stress, and antioxidant nutrients,” Toxicology, vol. 189, no. 1-2, pp. 147–163, 2003. View at Publisher · View at Google Scholar · View at Scopus
  9. I. Bremner, “Manifestations of copper excess,” The American Journal of Clinical Nutrition, vol. 67, no. 5, pp. 1069S–1073S, 1998. View at Google Scholar
  10. S. Shleev, J. Tkac, A. Christenson et al., “Direct electron transfer between copper-containing proteins and electrodes,” Biosensors and Bioelectronics, vol. 20, no. 12, pp. 2517–2554, 2005. View at Publisher · View at Google Scholar · View at Scopus
  11. S. Prabhu and E. K. Poulose, “Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects,” International Nano Letters, vol. 2, article 32, 2012. View at Publisher · View at Google Scholar
  12. M. Valko, H. Morris, and M. T. D. Cronin, “Metals, toxicity and oxidative stress,” Current Medicinal Chemistry, vol. 12, no. 10, pp. 1161–1208, 2005. View at Publisher · View at Google Scholar · View at Scopus
  13. L. Sintubin, W. De Windt, J. Dick et al., “Lactic acid bacteria as reducing and capping agent for the fast and efficient production of silver nanoparticles,” Applied Microbiology and Biotechnology, vol. 84, no. 4, pp. 741–749, 2009. View at Publisher · View at Google Scholar · View at Scopus
  14. C. Ning, X. Wang, L. Li et al., “Concentration ranges of antibacterial cations for showing the highest antibacterial efficacy but the least cytotoxicity against mammalian cells: implications for a new antibacterial mechanism,” Chemical Research in Toxicology, vol. 28, no. 9, pp. 1815–1822, 2015. View at Publisher · View at Google Scholar
  15. A. M. Studer, L. K. Limbach, L. Van Duc et al., “Nanoparticle cytotoxicity depends on intracellular solubility: comparison of stabilized copper metal and degradable copper oxide nanoparticles,” Toxicology Letters, vol. 197, no. 3, pp. 169–174, 2010. View at Publisher · View at Google Scholar · View at Scopus
  16. Z. Wang, N. Li, J. Zhao, J. C. White, P. Qu, and B. Xing, “CuO nanoparticle interaction with human epithelial cells: cellular uptake, location, export, and genotoxicity,” Chemical Research in Toxicology, vol. 25, no. 7, pp. 1512–1521, 2012. View at Publisher · View at Google Scholar · View at Scopus
  17. P. Cronholm, K. Midander, H. L. Karlsson, K. Elihn, I. O. Wallinder, and L. Möller, “Effect of sonication and serum proteins on copper release from copper nanoparticles and the toxicity towards lung epithelial cells,” Nanotoxicology, vol. 5, no. 2, pp. 269–281, 2011. View at Publisher · View at Google Scholar · View at Scopus
  18. C. Damm, H. Münstedt, and A. Rösch, “The antimicrobial efficacy of polyamide 6/silver-nano- and microcomposites,” Materials Chemistry and Physics, vol. 108, no. 1, pp. 61–66, 2008. View at Publisher · View at Google Scholar · View at Scopus
  19. A. Muñoz-Bonilla and M. Fernández-García, “Polymeric materials with antimicrobial activity,” Progress in Polymer Science, vol. 37, no. 2, pp. 281–339, 2012. View at Publisher · View at Google Scholar · View at Scopus
  20. G. Borkow and J. Gabbay, “Copper as a biocidal tool,” Current Medicinal Chemistry, vol. 12, no. 18, pp. 2163–2175, 2005. View at Publisher · View at Google Scholar · View at Scopus
  21. W. Zhang, Y.-H. Zhang, J.-H. Ji, J. Zhao, Q. Yan, and P. K. Chu, “Antimicrobial properties of copper plasma-modified polyethylene,” Polymer, vol. 47, no. 21, pp. 7441–7445, 2006. View at Publisher · View at Google Scholar · View at Scopus
  22. M. Grace, N. Chand, and S. K. Bajpai, “Copper alginate-cotton cellulose (CACC) fibers with excellent antibacterial properties,” Journal of Engineered Fibers and Fabrics, vol. 4, no. 3, pp. 1–14, 2009. View at Google Scholar
  23. W. Du, Y. Xu, Z. Xu, and C. Fan, “Preparation, characterization and antibacterial properties against E. coli K(88) of chitosan nanoparticle loaded copper ions,” Nanotechnology, vol. 19, no. 8, Article ID 085707, 2008. View at Publisher · View at Google Scholar
  24. P. Appendini and J. H. Hotchkiss, “Review of antimicrobial food packaging,” Innovative Food Science and Emerging Technologies, vol. 3, no. 2, pp. 113–126, 2002. View at Publisher · View at Google Scholar · View at Scopus
  25. C. Gunawan, W. Y. Teoh, C. P. Marquis, and R. Amal, “Cytotoxic origin of copper(II) oxide nanoparticles: comparative studies with micron-sized particles, leachate, and metal salts,” ACS Nano, vol. 5, no. 9, pp. 7214–7225, 2011. View at Publisher · View at Google Scholar · View at Scopus
  26. N. Cioffi, L. Torsi, N. Ditaranto et al., “Copper nanoparticle/polymer composites with antifungal and bacteriostatic properties,” Chemistry of Materials, vol. 17, no. 21, pp. 5255–5262, 2005. View at Publisher · View at Google Scholar · View at Scopus
  27. K. C. Anyaogu, A. V. Fedorov, and D. C. Neckers, “Synthesis, characterization, and antifouling potential of functionalized copper nanoparticles,” Langmuir, vol. 24, no. 8, pp. 4340–4346, 2008. View at Publisher · View at Google Scholar · View at Scopus
  28. G. Ren, D. Hu, E. W. C. Cheng, M. A. Vargas-Reus, P. Reip, and R. P. Allaker, “Characterisation of copper oxide nanoparticles for antimicrobial applications,” International Journal of Antimicrobial Agents, vol. 33, no. 6, pp. 587–590, 2009. View at Publisher · View at Google Scholar · View at Scopus
  29. H. Palza, S. Gutiérrez, K. Delgado et al., “Toward tailor-made biocide materials based on poly(propylene)/copper nanoparticles,” Macromolecular Rapid Communications, vol. 31, no. 6, pp. 563–567, 2010. View at Publisher · View at Google Scholar · View at Scopus
  30. K. Delgado, R. Quijada, R. Palma, and H. Palza, “Polypropylene with embedded copper metal or copper oxide nanoparticles as a novel plastic antimicrobial agent,” Letters in Applied Microbiology, vol. 53, no. 1, pp. 50–54, 2011. View at Publisher · View at Google Scholar · View at Scopus
  31. A. J. Bone, B. P. Colman, A. P. Gondikas et al., “Biotic and abiotic interactions in aquatic microcosms determine fate and toxicity of Ag nanoparticles: part 2–toxicity and Ag speciation,” Environmental Science & Technology, vol. 46, no. 13, pp. 6925–6933, 2012. View at Publisher · View at Google Scholar · View at Scopus
  32. S.-J. Yu, Y.-G. Yin, and J.-F. Liu, “Silver nanoparticles in the environment,” Environmental Science: Processes & Impacts, vol. 15, pp. 78–92, 2013. View at Publisher · View at Google Scholar
  33. D. Zhao, J. Feng, Q. Huo et al., “Triblock copolymer syntheses of mesoporous silica with periodic 50 to 300 angstrom pores,” Science, vol. 279, no. 5350, pp. 548–552, 1998. View at Publisher · View at Google Scholar · View at Scopus
  34. M. Laskowska, L. Laskowski, and J. Jelonkiewicz, “SBA-15 mesoporous silica activated by metal ions—verification of molecular structure on the basis of Raman spectroscopy supported by numerical simulations,” Journal of Molecular Structure, vol. 1100, Article ID 21662, pp. 21–26, 2015. View at Publisher · View at Google Scholar · View at Scopus
  35. L. Laskowski and M. Laskowska, “Functionalization of SBA-15 mesoporous silica by Cu-phosphonate units: probing of synthesis route,” Journal of Solid State Chemistry, vol. 220, pp. 221–226, 2014. View at Publisher · View at Google Scholar · View at Scopus
  36. Z. Wang, D. Wang, Z. Zhao, Y. Chen, and J. Lan, “A DFT study of the structural units in SBA-15 mesoporous molecular sieve,” Computational and Theoretical Chemistry, vol. 963, no. 2-3, pp. 403–411, 2011. View at Publisher · View at Google Scholar · View at Scopus
  37. P. Hohenberg and W. Kohn, “Inhomogeneous electron gas,” APS Journals Archive—Physical Review Letters, Physical Review, and Reviews of Modern Physics, vol. 136, no. 3, pp. B864–B871, 1964. View at Publisher · View at Google Scholar · View at Scopus
  38. W. Kohn and L. J. Sham, “Self-consistent equations including exchange and correlation effects,” Physical Review, vol. 140, no. 4A, pp. A1133–A1138, 1965. View at Publisher · View at Google Scholar · View at Scopus
  39. M. J. Frisch, G. W. Trucks, H. B. Schlegel et al., Gaussian09, Gaussian Inc, Wallingford, Conn, USA, 2009.
  40. M. H. Jamróz, J. C. Dobrowolski, and R. Brzozowski, “Vibrational modes of 2,6-, 2,7-, and 2,3-diisopropylnaphthalene. A DFT study,” Journal of Molecular Structure, vol. 787, no. 1–3, pp. 172–183, 2006. View at Publisher · View at Google Scholar · View at Scopus
  41. M. Vakili, S. F. Tayyari, M. Hakimi-Tabar, A.-R. Nekoei, and S. Kadkhodaei, “Structure and vibrational assignment of bis(benzoylacetonato)copper(II),” Journal of Molecular Structure, vol. 1058, pp. 308–317, 2014. View at Publisher · View at Google Scholar · View at Scopus
  42. R. Dennington, T. Keith, and J. Millam, GaussView Version 5, Semichem, Shawnee Mission, Kan, USA, 2009.
  43. M. Jamroz, “Vibrational Energy Distribution Analysis (VEDA): scopes and limitations,” Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, vol. 114, pp. 220–230, 2013. View at Publisher · View at Google Scholar
  44. M. Balouiri, M. Sadiki, and S. K. Ibnsouda, “Methods for in vitro evaluating antimicrobial activity: a review,” Journal of Pharmaceutical Analysis, vol. 6, no. 2, pp. 71–79, 2016. View at Publisher · View at Google Scholar · View at Scopus
  45. A. L. Barry, W. A. Craig, H. Nadler, L. B. Reller, C. C. Sanders, and J. M. Swenson, “Methods for determining bactericidal activity of antimicrobial agents-approved guideline,” NCCLS Document M26-A 19, NCCLS, Pennsylvania, Pa, USA, 1999. View at Google Scholar
  46. N. Das, H. Eckert, H. Hu, I. E. Wachs, J. F. Walzer, and F. J. Feher, “Bonding states of surface vanadium(V) oxide phases on silica: structural characterization by vanadium-51 NMR and Raman spectroscopy,” Journal of Physical Chemistry, vol. 97, no. 31, pp. 8240–8243, 1993. View at Publisher · View at Google Scholar · View at Scopus
  47. G. Du, S. Lim, M. Pinault et al., “Synthesis, characterization, and catalytic performance of highly dispersed vanadium grafted SBA-15 catalyst,” Journal of Catalysis, vol. 253, no. 1, pp. 74–90, 2008. View at Publisher · View at Google Scholar · View at Scopus
  48. K. Chamakura, R. Perez-Ballestero, Z. Luo, S. Bashir, and J. Liu, “Comparison of bactericidal activities of silver nanoparticles with common chemical disinfectants,” Colloids and Surfaces B: Biointerfaces, vol. 84, no. 1, pp. 88–96, 2011. View at Publisher · View at Google Scholar · View at Scopus
  49. K. Xu, J.-X. Wang, X.-L. Kang, and J.-F. Chen, “Fabrication of antibacterial monodispersed Ag–SiO2 core–shell nanoparticles with high concentration,” Materials Letters, vol. 63, no. 1, pp. 31–33, 2009. View at Publisher · View at Google Scholar · View at Scopus
  50. M. Ahamed, H. A. Alhadlaq, M. A. M. Khan, P. Karuppiah, and N. A. Al-Dhabi, “Synthesis, characterization, and antimicrobial activity of copper oxide nanoparticles,” Journal of Nanomaterials, vol. 2014, Article ID 637858, 4 pages, 2014. View at Publisher · View at Google Scholar · View at Scopus
  51. K. Helbig, C. Grosse, and D. H. Nies, “Cadmium toxicity in glutathione mutants of Escherichia coli,” Journal of Bacteriology, vol. 190, no. 15, pp. 5439–5454, 2008. View at Publisher · View at Google Scholar · View at Scopus
  52. E. R. Stadtman, “Oxidation of free amino acids and amino acid residues in proteins by radiolysis and by metal-catalyzed reactions,” Annual Review of Biochemistry, vol. 62, pp. 797–821, 1993. View at Publisher · View at Google Scholar · View at Scopus
  53. D. Touati, M. Jacques, B. Tardat, L. Bouchard, and S. Despied, “Lethal oxidative damage and mutagenesis are generated by iron in delta fur mutants of Escherichia coli: protective role of superoxide dismutase,” Journal of Bacteriology, vol. 177, no. 9, pp. 2305–2314, 1995. View at Publisher · View at Google Scholar · View at Scopus
  54. J. A. Imlay, “Pathways of oxidative damage,” Annual Review of Microbiology, vol. 57, pp. 395–418, 2003. View at Publisher · View at Google Scholar · View at Scopus
  55. G. M. Teitzel, A. Geddie, S. K. De Long, M. J. Kirisits, M. Whiteley, and M. R. Parsek, “Survival and growth in the presence of elevated copper: transcriptional profiling of copper-stressed Pseudomonas aeruginosa,” Journal of Bacteriology, vol. 188, no. 20, pp. 7242–7256, 2006. View at Publisher · View at Google Scholar · View at Scopus
  56. H. Palza, “Antimicrobial polymers with metal nanoparticles,” International Journal of Molecular Sciences, vol. 16, no. 1, pp. 2099–2116, 2015. View at Publisher · View at Google Scholar · View at Scopus
  57. J. P. Ruparelia, A. K. Chatterjee, S. P. Duttagupta, and S. Mukherji, “Strain specificity in antimicrobial activity of silver and copper nanoparticles,” Acta Biomaterialia, vol. 4, no. 3, pp. 707–716, 2008. View at Publisher · View at Google Scholar · View at Scopus
  58. G. R. Buettner and B. A. Jurkiewicz, “Catalytic metals, ascorbate and free radicals: combinations to avoid,” Radiation Research, vol. 145, no. 5, pp. 532–541, 1996. View at Publisher · View at Google Scholar · View at Scopus
  59. A. Gnatowski, J. Jelonkiewicz, Ł. Laskowski, and M. Laskowska, “Influence of the copper-containing SBA-15 silica fillers on the mechanical properties of high density polyethylene,” Journal of Nanomaterials, vol. 2016, Article ID 3291719, 8 pages, 2016. View at Publisher · View at Google Scholar · View at Scopus