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Journal of Nanomaterials
Volume 2017, Article ID 1352134, 8 pages
Research Article

Development of Paper Biosensor for the Detection of Phenol from Industrial Effluents Using Bioconjugate of Tyr-AuNps Mediated by Novel Isolate Streptomyces tuirus DBZ39

1A-DBT Research Laboratory, Department of Microbiology, Gulbarga University, Kalaburagi, Karnataka 585 106, India
2Department of Materials Science, Gulbarga University, Kalaburagi, Karnataka 585 106, India

Correspondence should be addressed to Bi Bi Zainab Mazhari; moc.liamg@baniazbm

Received 13 October 2016; Revised 14 January 2017; Accepted 24 January 2017; Published 26 April 2017

Academic Editor: Maria D. King

Copyright © 2017 Bi Bi Zainab Mazhari et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Paper biosensor was developed using Tyr-AuNps bioconjugate produced by Streptomyces for the detection of phenol from the effluent of wine, paper, and plastic industries. Among three filter papers assessed, Whatman number 2 filter paper was proved to be the best paper base for the development of biosensor. Tyrosinase and gold nanoparticles being produced by a single novel isolate Streptomyces tuirus DBZ39 proved to be efficient bioconjugate for the detection of phenol constituents, due to its biocompatibility. The substrate specific catalytic activity of the tyrosinase and unique Surface Plasmon Resonance attribute of gold nanoparticles are the cause for efficient detection of phenol constituents from the effluent of wine, paper, and plastic industries in 3 min. The different types and quantity of phenolic constituents in various industrial effluents, such as phenol in wine, dopamine in paper, and catechol in plastic effluents, were accurately detected by the bioconjugate. The efficacy of tyrosinase in the detection of phenol constituents was expected to be enhanced by the gold nanoparticles because of their electron, optical, and magnetic properties. This novel paper strip biosensor could be cost-effective and efficient means of future devices for the detection of phenolic pollutants from any environmental samples.