Structural and Chemical Characterization of Silica Spheres before and after Modification by Silanization for Trypsin Immobilization

Figure 1

Topographical images obtained by scanning electron microscopy (SEM) of control (a) and silanized silica surfaces (b) and atomic force microscopy (AFM) analysis (operating in contact mode) of control (c) and silanized silica (d). Graphical representation of roughness parameters results (, , , , , and ) related to control and silanized silica obtained by AFM (e). Force spectroscopy results obtained by force versus displacement curves analyses performed in an atomic force microscope operating in contact mode (f). indicates statistically significant difference ().

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